@article {64823,
	title = {GCIB-SEM: A path to 10 nm isotropic imaging of cubic millimeter volumes.},
	journal = {bioRxiv},
	year = {2019},
	month = {2019 Feb 28},
	abstract = {Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) generates 3D datasets optimally suited for segmentation of cell ultrastructure and automated connectome tracing but is limited to small fields of view and is therefore incompatible with the new generation of ultrafast multibeam SEMs. In contrast, section-based techniques are multibeam-compatible but are limited in z-resolution making automatic segmentation of cellular ultrastructure difficult. Here we demonstrate a novel 3D electron microscopy technique, Gas Cluster Ion Beam SEM (GCIB-SEM), in which top-down, wide-area ion milling is performed on a series of thick sections, acquiring < 10 nm isotropic datasets of each which are then stitched together to span the full sectioned volume. Based on our results, incorporating GCIB-SEM into existing single beam and multibeam SEM workflows should be straightforward and should dramatically increase reliability while simultaneously improving z-resolution by a factor of 3 or more.},
	doi = {10.1101/563239},
	author = {Hayworth, Kenneth J and Peale, David R and Januszewski, Michal and Knott, Graham and Lu, Zhiyuan and Xu, C. Shan and Hess, Harald F}
}
@article {1245,
	title = {Increasing depth resolution of electron microscopy of neural circuits using sparse tomographic reconstruction.},
	journal = {Computer Vision and Pattern Recognition (CVPR)},
	year = {2010},
	month = {2010},
	pages = {1767-74},
	issn = {1063-6919},
	doi = {10.1109/CVPR.2010.5539846},
	author = {Veeraraghavan, Ashok and Genkin, Alex V and Vitaladevuni, Shiv and Scheffer, Lou and Xu, CShan and Hess, Harald and Fetter, Richard and Cantoni, Marco and Knott, Graham and Chklovskii, Dmitri B}
}
@article {5253,
	title = {Rapid functional maturation of nascent dendritic spines.},
	journal = {Neuron},
	volume = {61},
	year = {2009},
	month = {2009 Jan 29},
	pages = {247-58},
	abstract = {<p>Spine growth and retraction with synapse formation and elimination plays an important role in shaping brain circuits during development and in the adult brain, yet the temporal relationship between spine morphogenesis and the formation of functional synapses remains poorly defined. We imaged hippocampal pyramidal neurons to identify spines of different ages. We then used two-photon glutamate uncaging, whole-cell recording, and Ca(2+) imaging to analyze the properties of nascent spines and their older neighbors. New spines expressed glutamate-sensitive currents that were indistinguishable from mature spines of comparable volumes. Some spines exhibited negligible AMPA receptor-mediated responses, but the occurrence of these "silent" spines was uncorrelated with spine age. In contrast, NMDA receptor-mediated Ca(2+) accumulations were significantly lower in new spines. New spines reconstructed using electron microscopy made synapses. Our data support a model in which outgrowth and enlargement of nascent spines is tightly coupled to formation and maturation of glutamatergic synapses.</p>
},
	keywords = {AMPA, Animals, Calcium, Calcium Signaling, Cell Differentiation, Confocal, Dendritic Spines, Electron, Glutamic Acid, Hippocampus, Microscopy, Neuronal Plasticity, Organ Culture Techniques, Patch-Clamp Techniques, Pyramidal Cells, Rats, Receptors, Synapses, Synaptic Membranes, Synaptic Transmission, Time Factors, Transmission},
	issn = {1097-4199},
	doi = {10.1016/j.neuron.2008.10.054},
	author = {Zito, Karen and Scheuss, Volker and Knott, Graham and Hill, Travis and Svoboda, Karel}
}
@article {6510,
	title = {Serial section scanning electron microscopy of adult brain tissue using focused ion beam milling.},
	journal = {The Journal of Neuroscience: The Official Journal of the Society for Neuroscience},
	volume = {28},
	year = {2008},
	month = {2008 Mar 19},
	pages = {2959-64},
	keywords = {Animals, Brain, Computer-Assisted, Electron, Female, Image Processing, Inbred C57BL, Ions, Mice, Microscopy, Scanning, Specimen Handling, Synapses},
	issn = {1529-2401},
	doi = {10.1523/JNEUROSCI.3189-07.2008},
	author = {Knott, Graham and Marchman, Herschel and Wall, David and Lich, Ben}
}
