@article {45699,
	title = {Photoswitchable red fluorescent protein with a large stokes shift.},
	journal = {Chemistry \& Biology},
	volume = {21},
	year = {2014},
	month = {2014 Oct 23},
	pages = {1402-14},
	abstract = {<p>A subclass of fluorescent proteins (FPs), large Stokes shift (LSS) FP, are characterized by increased spread between excitation and emission maxima. We report a photoswitchable variant of a red FP with an LSS, PSLSSmKate, which initially exhibits excitation and emission at 445 and 622\&nbsp;nm, but violet irradiation photoswitches PSLSSmKate into a common red form with excitation and emission at 573 and 621\&nbsp;nm. We characterize spectral, photophysical, and biochemical properties of PSLSSmKate in\&nbsp;vitro and in mammalian cells and determine its crystal structure in the LSS form. Mass spectrometry, mutagenesis, and spectroscopy of PSLSSmKate allow us\&nbsp;to propose molecular mechanisms for the LSS, pH dependence, and light-induced chromophore transformation. We demonstrate the applicability of PSLSSmKate to superresolution photoactivated localization microscopy and protein dynamics in live cells. Given its promising properties, we expect that PSLSSmKate-like phenotype will be further used for photoactivatable imaging and tracking multiple populations of intracellular objects.</p>
},
	issn = {1879-1301},
	doi = {10.1016/j.chembiol.2014.08.010},
	author = {Piatkevich, Kiryl D and English, Brian P and Malashkevich, Vladimir N and Xiao, Hui and Almo, Steven C and Singer, Robert H and Verkhusha, Vladislav V}
}
