@article {49060,
	title = {A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.},
	journal = {Nature Chemical Biology},
	year = {2018},
	month = {2018 Feb 26},
	abstract = {<p>We developed a new way to engineer complex proteins toward multidimensional specifications using a simple, yet scalable, directed evolution strategy. By robotically picking mammalian cells that were identified, under a microscope, as expressing proteins that simultaneously exhibit several specific properties, we can screen hundreds of thousands of proteins in a library in just a few hours, evaluating each along multiple performance axes. To demonstrate the power of this approach, we created\&nbsp;a genetically encoded fluorescent voltage indicator, simultaneously optimizing its\&nbsp;brightness and membrane localization using our microscopy-guided cell-picking strategy. We produced the high-performance opsin-based fluorescent voltage reporter Archon1 and demonstrated its utility by imaging spiking and millivolt-scale subthreshold and synaptic activity in acute mouse brain slices and in larval zebrafish in vivo. We also measured postsynaptic responses downstream of optogenetically controlled neurons in C. elegans.</p>
},
	issn = {1552-4469},
	doi = {10.1038/s41589-018-0004-9},
	author = {Piatkevich, Kiryl D and Jung, Erica E and Straub, Christoph and Linghu, Changyang and Park, Demian and Suk, Ho-Jun and Hochbaum, Daniel R and Goodwin, Daniel and Pnevmatikakis, Eftychios and Pak, Nikita and Kawashima, Takashi and Yang, Chao-Tsung and Rhoades, Jeffrey L and Shemesh, Or and Asano, Shoh and Yoon, Young-Gyu and Freifeld, Limor and Saulnier, Jessica L and Riegler, Clemens and Engert, Florian and Hughes, Thom and Drobizhev, Mikhail and Szabo, Balint and Ahrens, Misha B and Flavell, Steven W and Sabatini, Bernardo L and Boyden, Edward S}
}
@article {46050,
	title = {Distinct functions of kainate receptors in the brain are determined by the auxiliary subunit Neto1.},
	journal = {Nature Neuroscience},
	volume = {14},
	year = {2011},
	month = {2011 Jul},
	pages = {866-73},
	abstract = {<p>Ionotropic glutamate receptors principally mediate fast excitatory transmission in the brain. Among the three classes of ionotropic glutamate receptors, kainate receptors (KARs) have a unique brain distribution, which has been historically defined by (3)H-radiolabeled kainate binding. Compared with recombinant KARs expressed in heterologous cells, synaptic KARs exhibit characteristically slow rise-time and decay kinetics. However, the mechanisms responsible for these distinct KAR properties remain unclear. We found that both the high-affinity binding pattern in the mouse brain and the channel properties of native KARs are determined by the KAR auxiliary subunit Neto1. Through modulation of agonist binding affinity and off-kinetics of KARs, but not trafficking of KARs, Neto1 determined both the KAR high-affinity binding pattern and the distinctively slow kinetics of postsynaptic KARs. By regulating KAR excitatory postsynaptic current kinetics, Neto1 can control synaptic temporal summation, spike generation and fidelity.</p>
},
	keywords = {Animals, Animals, Newborn, Biophysical Phenomena, Biophysics, CA1 Region, Hippocampal, Cell Line, Transformed, Cerebellum, Dizocilpine Maleate, Dose-Response Relationship, Drug, Drug Interactions, Electric Stimulation, Excitatory Amino Acid Agonists, Excitatory Amino Acid Antagonists, Excitatory Postsynaptic Potentials, Gene Expression Regulation, Green Fluorescent Proteins, Guanylate Kinase, Humans, Immunoprecipitation, In Vitro Techniques, Intracellular Signaling Peptides and Proteins, Kainic Acid, Lipoproteins, LDL, Membrane Potentials, Membrane Proteins, Mice, Mice, Knockout, Neurons, Patch-Clamp Techniques, Presynaptic Terminals, Protein Binding, Protein Subunits, Receptors, Kainic Acid, Synaptophysin, Transfection, Tritium},
	issn = {1546-1726},
	doi = {10.1038/nn.2837},
	author = {Straub, Christoph and Hunt, David L and Yamasaki, Miwako and Kim, Kwang S and Watanabe, Masahiko and Castillo, Pablo E and Tomita, Susumu}
}
