@article {65224,
	title = {Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution.},
	journal = {Nature Methods},
	volume = {17},
	year = {2019},
	month = {2020 Jan 01},
	pages = {68-71},
	abstract = {<p>We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with \&lt;10 nm isotropic resolution of each section, and these can then be stitched together to span the sectioned volume. Incorporating gas cluster ion beam SEM into existing single-beam and multibeam SEM workflows should be straightforward, increasing reliability while improving z resolution by a factor of three or more.</p>
},
	issn = {1548-7105},
	doi = {10.1038/s41592-019-0641-2},
	author = {Hayworth, Kenneth J and Peale, David and Januszewski, Micha{\l} and Knott, Graham W and Lu, Zhiyuan and Xu, C Shan and Hess, Harald F}
}
@article {48254,
	title = {Multicut brings automated neurite segmentation closer to human performance.},
	journal = {Nature Methods},
	volume = {14},
	year = {2017},
	month = {2017 Jan 31},
	pages = {101-102},
	issn = {1548-7105},
	doi = {10.1038/nmeth.4151},
	author = {Beier, Thorsten and Pape, Constantin and Rahaman, Nasim and Prange, Timo and Berg, Stuart and Bock, Davi D and Cardona, Albert and Knott, Graham W and Plaza, Stephen M and Scheffer, Louis K and Koethe, Ullrich and Kreshuk, Anna and Hamprecht, Fred A}
}
@article {46325,
	title = {Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics.},
	journal = {Nature Methods},
	volume = {12},
	year = {2015},
	month = {2015 Feb 16},
	pages = {319-22},
	abstract = {<p>Focused-ion-beam scanning electron microscopy (FIB-SEM) has become an essential tool for studying neural tissue at resolutions below 10 nm {\texttimes} 10 nm {\texttimes} 10 nm, producing data sets optimized for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 μm thick) optimally sized and mounted for efficient, parallel FIB-SEM imaging. These chunks are imaged separately and then {\textquoteright}volume stitched{\textquoteright} back together, producing a final three-dimensional data set suitable for connectome tracing.</p>
},
	issn = {1548-7105},
	doi = {10.1038/nmeth.3292},
	author = {Hayworth, Kenneth J and Xu, C Shan and Lu, Zhiyuan and Knott, Graham W and Fetter, Richard D and Tapia, Juan Carlos and Lichtman, Jeff W and Hess, Harald F}
}
