@article {68812,
	title = {A viral toolbox for conditional and transneuronal gene expression in zebrafish},
	journal = {eLife},
	volume = {11},
	year = {2022},
	month = {07/2022},
	pages = {e77153},
	abstract = {<p>The zebrafish is an important model in systems neuroscience but viral tools to dissect the structure and function of neuronal circuitry are not established. We developed methods for efficient gene transfer and retrograde tracing in adult and larval zebrafish by herpes simplex viruses (HSV1). HSV1 was combined with the Gal4/UAS system to target cell types with high spatial, temporal, and molecular specificity. We also established methods for efficient transneuronal tracing by modified rabies viruses in zebrafish. We demonstrate that HSV1 and rabies viruses can be used to visualize and manipulate genetically or anatomically identified neurons within and across different brain areas of adult and larval zebrafish. An expandable library of viruses is provided to express fluorescent proteins, calcium indicators, optogenetic probes, toxins and other molecular tools. This toolbox creates new opportunities to interrogate neuronal circuits in zebrafish through combinations of genetic and viral approaches.</p>
},
	keywords = {Gene Transfer, Imaging, neuronal circuit, viral vector},
	issn = {2050-084X},
	doi = {10.7554/eLife.77153},
	url = {https://doi.org/10.7554/eLife.77153},
	author = {Satou, Chie and Neve, Rachael L and Oyibo, Hassana K and Zmarz, Pawel and Huang, Kuo-Hua and Arn Bouldoires, Estelle and Mori, Takuma and Higashijima, Shin-Ichi and Keller, Georg B and Friedrich, Rainer W},
	editor = {Bagnall, Martha W and Stainier, Didier YR and Jin, Lei}
}
@article {6222,
	title = {Spinal projection neurons control turning behaviors in zebrafish.},
	journal = {Current Biology},
	volume = {23},
	year = {2013},
	month = {2013 Aug 19},
	pages = {1566-73},
	abstract = {<p>Discrete populations of brainstem spinal projection neurons (SPNs) have been shown to exhibit behavior-specific responses during locomotion [1-9], suggesting that separate descending pathways, each dedicated to a specific behavior, control locomotion. In an alternative model, a large variety of motor outputs could be generated from different combinations of a small number of basic motor pathways. We examined this possibility by studying the precise role of ventromedially located hindbrain SPNs (vSPNs) in generating turning behaviors. We found that unilateral laser ablation of vSPNs reduces the tail deflection and cycle period specifically during the first undulation cycle of a swim bout, whereas later tail movements are unaffected. This holds true during phototaxic [10], optomotor [11], dark-flash-induced [12], and spontaneous turns [13], suggesting a universal role of these neurons in controlling turning behaviors. Importantly, we found that the ablation not only abolishes turns but also results in a dramatic increase in the number of forward swims, suggesting that these neurons transform forward swims into turns by introducing turning kinematics into a basic motor pattern of symmetric tail undulations. Finally, we show that vSPN activity is direction specific and graded by turning angle. Together, these results provide a clear example of how a specific motor pattern can be transformed into different behavioral events by the graded activation of a small set of SPNs.</p>
},
	issn = {1879-0445},
	doi = {10.1016/j.cub.2013.06.044},
	author = {Huang, Kuo-Hua and Ahrens, Misha B and Dunn, Timothy W. and Engert, Florian}
}
