@article {46707,
	title = {Direct observation of frequency modulated transcription in single cells using light activation.},
	journal = {eLife},
	volume = {2},
	year = {2013},
	month = {2013 Sep 24},
	pages = {e00750},
	abstract = {<p>Single-cell analysis has revealed that transcription is dynamic and stochastic, but tools are lacking that can determine the mechanism operating at a single gene. Here we utilize single-molecule observations of RNA in fixed and living cells to develop a single-cell model of steroid-receptor mediated gene activation. We determine that steroids drive mRNA synthesis by frequency modulation of transcription. This digital behavior in single cells gives rise to the well-known analog dose response across the population. To test this model, we developed a light-activation technology to turn on a single steroid-responsive gene and follow dynamic synthesis of RNA from the activated locus. DOI:http://dx.doi.org/10.7554/eLife.00750.001.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.00750},
	author = {Larson, Daniel R and Fritzsch, Christoph and Sun, Liang and Meng, Xiuhau and Lawrence, David S and Singer, Robert H}
}
