@article {46736,
	title = {Cotranscriptional effect of a premature termination codon revealed by live-cell imaging.},
	journal = {RNA},
	volume = {17},
	year = {2011},
	month = {2011 Dec},
	pages = {2094-107},
	abstract = {<p>Aberrant mRNAs with premature translation termination codons (PTCs) are recognized and eliminated by the nonsense-mediated mRNA decay (NMD) pathway in eukaryotes. We employed a novel live-cell imaging approach to investigate the kinetics of mRNA synthesis and release at the transcription site of PTC-containing (PTC+) and PTC-free (PTC-) immunoglobulin-μ reporter genes. Fluorescence recovery after photobleaching (FRAP) and photoconversion analyses revealed that PTC+ transcripts are specifically retained at the transcription site. Remarkably, the retained PTC+ transcripts are mainly unspliced, and this RNA retention is dependent upon two important NMD factors, UPF1 and SMG6, since their depletion led to the release of the PTC+ transcripts. Finally, ChIP analysis showed a physical association of UPF1 and SMG6 with both the PTC+ and the PTC- reporter genes in vivo. Collectively, our data support a mechanism for regulation of PTC+ transcripts at the transcription site.</p>
},
	keywords = {Cell Line, Codon, Nonsense, Cytoplasm, Gene Expression Regulation, Gene Order, Gene Silencing, Humans, Immunoglobulin mu-Chains, In Situ Hybridization, Fluorescence, Nonsense Mediated mRNA Decay, RNA Splicing, RNA, Messenger, Telomerase, Trans-Activators, Transcription, Genetic},
	issn = {1469-9001},
	doi = {10.1261/rna.02918111},
	author = {de Turris, Valeria and Nicholson, Pamela and Orozco, Rodolfo Zamudio and Singer, Robert H and M{\"u}hlemann, Oliver}
}
