@article {46774,
	title = {Actin depletion initiates events leading to granule secretion at the immunological synapse.},
	journal = {Immunity},
	volume = {42},
	year = {2015},
	month = {2015 May 19},
	pages = {864-76},
	abstract = {<p>Cytotoxic T lymphocytes (CTLs) use polarized secretion to rapidly destroy virally infected and tumor cells. To understand the temporal relationships between key events leading to secretion, we used high-resolution 4D imaging. CTLs approached targets with actin-rich projections at the leading edge, creating an initially actin-enriched contact with rearward-flowing actin. Within 1\&nbsp;min, cortical actin reduced across the synapse, T\&nbsp;cell receptors (TCRs) clustered centrally to form the central supramolecular activation cluster (cSMAC), and centrosome polarization began. Granules clustered around the moving centrosome within 2.5\&nbsp;min and reached the synapse after 6\&nbsp;min. TCR-bearing intracellular vesicles were delivered to the cSMAC as the centrosome docked. We found that the centrosome and granules were delivered to an area of membrane with reduced cortical actin density and phospholipid PIP2. These data resolve the temporal order of events during synapse maturation in 4D and reveal a critical role for actin depletion in regulating secretion.</p>
},
	issn = {1097-4180},
	doi = {10.1016/j.immuni.2015.04.013},
	author = {Ritter, Alex T and Asano, Yukako and Stinchcombe, Jane C and Dieckmann, N M G and Chen, Bi-Chang and Gawden-Bone, C and van Engelenburg, Schuyler and Legant, Wesley and Gao, Liang and Davidson, Michael W and Betzig, Eric and Lippincott-Schwartz, Jennifer and Griffiths, Gillian M}
}
@article {47655,
	title = {Visualizing cell structure and function with point-localization superresolution imaging.},
	journal = {Developmental cell},
	volume = {23},
	year = {2012},
	month = {2012 Dec 11},
	pages = {1092-102},
	abstract = {<p>Fundamental to the success of cell and developmental biology is the ability to tease apart molecular organization in cells and tissues by localizing specific proteins with respect to one another in a native cellular context. However, many key cellular structures (from mitochondrial cristae to nuclear pores) lie below the diffraction limit of visible light, precluding analysis of their organization by conventional approaches. Point-localization superresolution microscopy techniques, such as PALM and STORM, are poised to resolve, with unprecedented clarity, the organizational principles of macromolecular complexes within cells, thus leading to deeper insights into cellular function in both health and disease.</p>
},
	keywords = {Cell Physiological Phenomena, Cells, Cellular Structures, Fluorescent Dyes, Imaging, Three-Dimensional, Microscopy, Microscopy, Fluorescence, Mitochondrial Membranes},
	issn = {1878-1551},
	doi = {10.1016/j.devcel.2012.09.022},
	author = {Sengupta, Prabuddha and van Engelenburg, Schuyler and Lippincott-Schwartz, Jennifer}
}
