@article {47307,
	title = {Transcriptomes of lineage-specific Drosophila neuroblasts profiled by genetic targeting and robotic sorting.},
	journal = {Development (Cambridge, England)},
	volume = {143},
	year = {2016},
	month = {2016 Feb 1},
	pages = {411-21},
	abstract = {<p>A brain consists of numerous distinct neurons arising from a limited number of progenitors, called neuroblasts in Drosophila. Each neuroblast produces a specific neuronal lineage. To unravel the transcriptional networks that underlie the development of distinct neuroblast lineages, we marked and isolated lineage-specific neuroblasts for RNA sequencing. We labeled particular neuroblasts throughout neurogenesis by activating a conditional neuroblast driver in specific lineages using various intersection strategies. The targeted neuroblasts were efficiently recovered using a custom-built device for robotic single-cell picking. Transcriptome analysis of mushroom body, antennal lobe and type II neuroblasts compared with non-selective neuroblasts, neurons and glia revealed a rich repertoire of transcription factors expressed among neuroblasts in diverse patterns. Besides transcription factors that are likely to be pan-neuroblast, many transcription factors exist that are selectively enriched or repressed in certain neuroblasts. The unique combinations of transcription factors present in different neuroblasts may govern the diverse lineage-specific neuron fates.</p>
},
	issn = {1477-9129},
	doi = {10.1242/dev.129163},
	author = {Yang, Ching-Po and Fu, Chi-Cheng and Sugino, Ken and Liu, Zhiyong and Ren, Qingzhong and Liu, Ling-Yu and Yao, Xiaohao and Lee, Luke P and Lee, Tzumin}
}
@article {47126,
	title = {Opposing intrinsic temporal gradients guide neural stem cell production of varied neuronal fates.},
	journal = {Science (New York, N.Y.)},
	volume = {350},
	year = {2015},
	month = {2015 Oct 16},
	pages = {317-20},
	abstract = {<p>Neural stem cells show age-dependent developmental potentials, as evidenced by their production of distinct neuron types at different developmental times. Drosophila neuroblasts produce long, stereotyped lineages of neurons. We searched for factors that could regulate neural temporal fate by RNA-sequencing lineage-specific neuroblasts at various developmental times. We found that two RNA-binding proteins, IGF-II mRNA-binding protein (Imp) and Syncrip (Syp), display opposing high-to-low and low-to-high temporal gradients with lineage-specific temporal dynamics. Imp and Syp promote early and late fates, respectively, in both a slowly progressing and a rapidly changing lineage. Imp and Syp control neuronal fates in the mushroom body lineages by regulating the temporal transcription factor Chinmo translation. Together, the opposing Imp/Syp gradients encode stem cell age, specifying multiple cell fates within a lineage.</p>
},
	issn = {1095-9203},
	doi = {10.1126/science.aad1886},
	author = {Liu, Zhiyong and Yang, Ching-Po and Sugino, Ken and Fu, Chi-Cheng and Liu, Ling-Yu and Yao, Xiaohao and Lee, Luke P and Lee, Tzumin}
}
