@article {47729,
	title = {Bright photoactivatable fluorophores for single-molecule imaging.},
	journal = {Nature Methods},
	volume = {13},
	year = {2016},
	month = {2016 Oct 24},
	pages = {985-8},
	abstract = {<p>Small molecule fluorophores are important tools for advanced imaging experiments. The development of self-labeling protein tags such as the HaloTag and SNAP-tag has expanded the utility of chemical dyes in live-cell microscopy. We recently described a general method for improving the brightness and photostability of small, cell-permeable fluorophores, resulting in the novel azetidine-containing "Janelia Fluor" (JF) dyes. Here, we refine and extend the utility of the JF dyes by synthesizing photoactivatable derivatives that are compatible with live cell labeling strategies. These compounds retain the superior brightness of the JF dyes once activated, but their facile photoactivation also enables improved single-particle tracking and localization microscopy experiments.</p>
},
	doi = {10.1038/nmeth.4034},
	author = {Lavis, Luke D and Grimm, Jonathan B and English, Brian P and Choi, Heejin and Muthusamy, Anand K and Mehl, Brian P and Dong, Peng and Brown, Timothy A and Lippincott-Schwartz, Jennifer and Liu, Zhe and Lionnet, Timoth{\'e}e}
}
@article {47133,
	title = {Stochastic electrotransport selectively enhances the transport of highly electromobile molecules.},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {112},
	year = {2015},
	month = {2015 Nov 2},
	pages = {E6274-83},
	abstract = {<p>Nondestructive chemical processing of porous samples such as fixed biological tissues typically relies on molecular diffusion. Diffusion into a porous structure is a slow process that significantly delays completion of chemical processing. Here, we present a novel electrokinetic method termed stochastic electrotransport for rapid nondestructive processing of porous samples. This method uses a rotational electric field to selectively disperse highly electromobile molecules throughout a porous sample without displacing the low-electromobility molecules that constitute the sample. Using computational models, we show that stochastic electrotransport can rapidly disperse electromobile molecules in a porous medium. We apply this method to completely clear mouse organs within 1{\textendash}3 days and to stain them with nuclear dyes, proteins, and antibodies within 1 day. Our results demonstrate the potential of stochastic electrotransport to process large and dense tissue samples that were previously infeasible in time when relying on diffusion.</p>
},
	doi = {10.1073/pnas.1510133112},
	author = {Kim, Sung-Yon and Cho, Jae Hun and Murray, Evan and Bakh, Naveed and Choi, Heejin and Ohn, Kimberly and Ruelas, Luzdary and Hubbert, Austin and McCue, Meg and Vassallo, Sara L and Keller, Philipp J and Chung, Kwanghun}
}
