@article {6133,
	title = {Functional labeling of neurons and their projections using the synthetic activity-dependent promoter E-SARE.},
	journal = {Nature Methods},
	volume = {10},
	year = {2013},
	month = {2013 Jul 14},
	pages = {889-95},
	abstract = {<p>Identifying the neuronal ensembles that respond to specific stimuli and mapping their projection patterns in living animals are fundamental challenges in neuroscience. To this end, we engineered a synthetic promoter, the enhanced synaptic activity-responsive element (E-SARE), that drives neuronal activity-dependent gene expression more potently than other existing immediate-early gene promoters. Expression of a drug-inducible Cre recombinase downstream of E-SARE enabled imaging of neuronal populations that respond to monocular visual stimulation and tracking of their long-distance thalamocortical projections in living mice. Targeted cell-attached recordings and calcium imaging of neurons in sensory cortices revealed that E-SARE reporter expression correlates with sensory-evoked neuronal activity at the single-cell level and is highly specific to the type of stimuli presented to the animals. This activity-dependent promoter can expand the repertoire of genetic approaches for high-resolution anatomical and functional analysis of neural circuits.</p>
},
	issn = {1548-7105},
	doi = {10.1038/nmeth.2559},
	author = {Kawashima, Takashi and Kitamura, Kazuo and Suzuki, Kanzo and Nonaka, Mio and Kamijo, Satoshi and Takemoto-Kimura, Sayaka and Kano, Masanobu and Okuno, Hiroyuki and Ohki, Kenichi and Bito, Haruhiko}
}
