@article {49478,
	title = {Correlated evolution of two copulatory organs via a single cis-regulatory nucleotide change.},
	journal = {Current Biology : CB},
	volume = {28},
	year = {2018},
	month = {2018 Oct 05},
	pages = {3450-7},
	abstract = {<p>Diverse traits often covary between species [1-3]. The possibility that a single mutation could contribute to the evolution of several characters between species [3] is rarely investigated as relatively few cases are dissected at the nucleotide level. Drosophila santomea has evolved additional sex comb sensory teeth on its legs and has lost two sensory bristles on its genitalia. We present evidence that a single nucleotide substitution in an enhancer of the scute gene contributes to both changes. The mutation alters a binding site for the Hox protein Abdominal-B in the developing genitalia, leading to bristle loss, and for another factor in the developing leg, leading to bristle gain. Our study suggests that morphological evolution between species can occur through a single nucleotide change affecting several sexually dimorphic traits. VIDEO ABSTRACT.</p>
},
	issn = {1879-0445},
	doi = {10.1016/j.cub.2018.08.047},
	author = {Nagy, Olga and Nuez, Isabelle and Savisaar, Rosina and Peluffo, Alexandre E and Yassin, Amir and Lang, Michael and Stern, David L and Matute, Daniel R and David, Jean R and Courtier-Orgogozo, Virginie}
}
@article {47238,
	title = {A major locus controls a genital shape difference involved in reproductive isolation between Drosophila yakuba and Drosophila santomea.},
	journal = {G3 (Bethesda, Md.)},
	volume = {5},
	year = {2015},
	month = {2015 Oct 28},
	pages = {2893-901},
	abstract = {<p>Rapid evolution of genitalia shape, a widespread phenomenon in animals with internal fertilization, offers the opportunity to dissect the genetic architecture of morphological evolution linked to sexual selection and speciation. Most quantitative trait loci (QTL) mapping studies of genitalia divergence have focused on Drosophila melanogaster and its three most closely related species, D. simulans, D. mauritiana, and D. sechellia, and have suggested that the genetic basis of genitalia evolution involves many loci. We report the first genetic study of male genitalia evolution between D. yakuba and D. santomea, two species of the D. melanogaster species subgroup. We focus on male ventral branches, which harm females during interspecific copulation. Using landmark-based geometric morphometrics, we characterized shape variation in parental species, F1 hybrids, and backcross progeny and show that the main axis of shape variation within the backcross population matches the interspecific variation between parental species. For genotyping, we developed a new molecular method to perform multiplexed shotgun genotyping (MSG), which allowed us to prepare genomic DNA libraries from 365 backcross individuals in a few days using little DNA. We detected only three QTL, one of which spans 2.7 Mb and exhibits a highly significant effect on shape variation that can be linked to the harmfulness of the ventral branches. We conclude that the genetic architecture of genitalia morphology divergence may not always be as complex as suggested by previous studies.</p>
},
	issn = {2160-1836},
	doi = {10.1534/g3.115.023481},
	author = {Peluffo, Alexandre E and Nuez, Isabelle and Debat, Vincent and Savisaar, Rosina and Stern, David L and Orgogozo, Virginie}
}
