@article {65697,
	title = {Cortical RORβ is required for layer 4 transcriptional identity and barrel integrity.},
	journal = {eLife},
	volume = {9},
	year = {2020},
	month = {2020 Aug 27},
	abstract = {<p>Retinoic Acid-Related Orphan Receptor Beta (RORβ) is a transcription factor (TF) and marker of layer 4 (L4) neurons, which are distinctive both in transcriptional identity and the ability to form aggregates such as barrels in rodent somatosensory cortex. However, the relationship between transcriptional identity and L4 cytoarchitecture is largely unknown. We find RORβ is required in the cortex for L4 aggregation into barrels and thalamocortical afferent (TCA) segregation. Interestingly, barrel organization also degrades with age in wildtype mice. Loss of RORβ delays excitatory input and disrupts gene expression and chromatin accessibility, with down-regulation of L4 and up-regulation of L5 genes, suggesting a disruption in cellular specification. Expression and binding site accessibility change for many other TFs, including closure of neurodevelopmental TF binding sites and increased expression and binding capacity of activity-regulated TFs. Lastly, a putative target of RORβ, , is down-regulated without RORβ, and knock-out alone disrupts TCA organization in adult barrels.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.52370},
	author = {Clark, Erin A and Rutlin, Michael and Capano, Lucia and Aviles, Samuel and Saadon, Jordan R and Taneja, Praveen and Zhang, Qiyu and Bullis, James B and Lauer, Timothy and Myers, Emma and Schulmann, Anton and Forrest, Douglas and Nelson, Sacha B}
}
@article {47383,
	title = {A mammalian enhancer trap resource for discovering and manipulating neuronal cell types.},
	journal = {eLife},
	volume = {5},
	year = {2016},
	month = {2016 Mar 21},
	abstract = {<p>There is a continuing need for driver strains to enable cell type-specific manipulation in the nervous system. Each cell type expresses a unique set of genes, and recapitulating expression of marker genes by BAC transgenesis or knock-in has generated useful transgenic mouse lines. However since genes are often expressed in many cell types, many of these lines have relatively broad expression patterns. We report an alternative transgenic approach capturing distal enhancers for more focused expression. We identified an enhancer trap probe often producing restricted reporter expression and developed efficient enhancer trap screening with the PiggyBac transposon. We established more than 200 lines and found many lines that label small subsets of neurons in brain substructures, including known and novel cell types. Images and other information about each line are available online (enhancertrap.bio.brandeis.edu).</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.13503},
	author = {Shima, Yasuyuki and Sugino, Ken and Hempel, Chris and Shima, Masami and Taneja, Praveen and Bullis, James B and Mehta, Sonam and Lois, Carlos and Nelson, Sacha B}
}
