@article {67803,
	title = {De novo endocytic clathrin coats develop curvature at early stages of their formation.},
	journal = {Development Cell},
	volume = {56},
	year = {2021},
	month = {2021 Nov 22},
	pages = {3146-3159.e5},
	abstract = {<p>Sculpting a flat patch of membrane into an endocytic vesicle requires curvature generation on the cell surface, which is the primary function of the endocytosis machinery. Using super-resolved live cell fluorescence imaging, we demonstrate that curvature generation by individual clathrin-coated pits can be detected in real time within cultured cells and tissues of developing organisms. Our analyses demonstrate that the footprint of clathrin coats increases monotonically during the formation of pits at different levels of plasma membrane tension. These findings are only compatible with models that predict curvature generation at the early stages of endocytic clathrin pit formation. We also found that CALM adaptors associated with clathrin plaques form clusters, whereas AP2 distribution is more homogenous. Considering the curvature sensing and driving roles of CALM, we propose that CALM clusters may increase the strain on clathrin lattices locally, eventually giving rise to rupture and subsequent pit completion at the edges of plaques.</p>
},
	issn = {1878-1551},
	doi = {10.1016/j.devcel.2021.10.019},
	author = {Willy, Nathan M and Ferguson, Joshua P and Akatay, Ata and Huber, Scott and Djakbarova, Umidahan and Silahli, Salih and Cakez, Cemal and Hasan, Farah and Chang, Henry C and Travesset, Alex and Li, Siyu and Zandi, Roya and Li, Dong and Betzig, Eric and Cocucci, Emanuele and Kural, Comert}
}
@article {47790,
	title = {Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy.},
	journal = {Molecular Biology of the Cell},
	volume = {27},
	year = {2016},
	month = {2016 Aug 17},
	pages = {3418-35},
	abstract = {<p>Membrane remodeling is an essential part for transfer of components to and from the cell surface and membrane-bound organelles, and for changes in cell shape, particularly critical during cell division. Earlier analyses, based on classical optical live-cell imaging, mostly restricted by technical necessity to the attached bottom surface, showed persistent formation of endocytic clathrin pits and vesicles during mitosis. Taking advantage of the resolution, speed, and non-invasive illumination of the newly developed lattice light sheet fluorescence microscope, we reexamined their assembly dynamics over the entire cell surface and showed that clathrin pits form at a lower rate during late mitosis. Full-cell imaging measurements of cell surface area and volume throughout the cell cycle of single cells in culture and in zebrafish embryos showed that the total surface increased rapidly during the transition from telophase to cytokinesis, whereas cell volume increased slightly in metaphase and remained relatively constant during cytokinesis. These applications demonstrate the advantage of lattice light sheet microscopy and enable a new standard for imaging membrane dynamics in single cells and in multicellular assemblies.</p>
},
	issn = {1939-4586},
	doi = {10.1091/mbc.E16-03-0164},
	author = {Aguet, Fran{\c c}ois and Upadhyayula, Srigokul and Gaudin, Rapha{\"e}l and Chou, Yi-ying and Cocucci, Emanuele and He, Kangmin and Chen, Bi-Chang and Mosaliganti, Kishore and Pasham, Mithun and Skillern, Wesley and Legant, Wesley R and Liu, Tsung-Li and Findlay, Greg and Marino, Eric and Danuser, Gaudenz and Megason, Sean and Betzig, Eric and Kirchhausen, Tom}
}
