@article {6594,
	title = {Real-time dynamics of RNA polymerase II clustering in live human cells.},
	journal = {Science},
	volume = {341},
	year = {2013},
	month = {2013 Aug 9},
	pages = {664-7},
	abstract = {<p>Transcription is reported to be spatially compartmentalized in nuclear transcription factories with clusters of RNA polymerase II (Pol II). However, little is known about when these foci assemble or their relative stability. We developed a quantitative single-cell approach to characterize protein spatiotemporal organization, with single-molecule sensitivity in live eukaryotic cells. We observed that Pol II clusters form transiently, with an average lifetime of 5.1 ({\textpm} 0.4) seconds, which refutes the notion that they are statically assembled substructures. Stimuli affecting transcription yielded orders-of-magnitude changes in the dynamics of Pol II clusters, which implies that clustering is regulated and plays a role in the cell{\textquoteright}s ability to effect rapid response to external signals. Our results suggest that transient crowding of enzymes may aid in rate-limiting steps of gene regulation.</p>
},
	keywords = {Cell Line, Flavonoids, Gene Expression Regulation, Genetic, Humans, Piperidines, RNA Polymerase II, Single-Cell Analysis, Time Factors, Transcription, Transcription Elongation, Tumor},
	issn = {1095-9203},
	doi = {10.1126/science.1239053},
	author = {Cisse, Ibrahim I and Izeddin, Ignacio and Causse, Sebastien Z and Boudarene, Lydia and Senecal, Adrien and Muresan, Leila and Dugast-Darzacq, Claire and Hajj, Bassam and Dahan, Maxime and Darzacq, Xavier}
}
@article {2390,
	title = {Imaging transcription in living cells.},
	journal = {Annual Review of Biophysics},
	volume = {38},
	year = {2009},
	month = {2009 Feb 3},
	pages = {173-96},
	abstract = {<p>The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene. We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.</p>
},
	keywords = {Computer-Assisted, Fluorescence, Gene Expression Profiling, Image Interpretation, Microscopy, Transcription Factors, Transcriptional Activation},
	issn = {1936-122X},
	doi = {10.1073/pnas.1100640108},
	author = {Darzacq, Xavier and Yao, Jie and Larson, Daniel R and Causse, Sebastien Z and Bosanac, Lana and de Turris, Valeria and Ruda, Vera M and Lionnet, Timoth{\'e}e and Zenklusen, Daniel and Guglielmi, Benjamin and Tjian, Robert and Singer, Robert H}
}
