@article {65758,
	title = {Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina.},
	journal = {eLife},
	volume = {9},
	year = {2020},
	month = {2020 Oct 06},
	abstract = {<p>An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly in time - and suggested crowding from cell division at the apical surface drives basalward motion. Yet, this hypothesis has not yet been tested, and the forces involved not quantified. We employ long-term, rapid light-sheet and two-photon imaging of early zebrafish retinogenesis to track entire populations of nuclei within the tissue. The time-varying concentration profiles show clear evidence of crowding as nuclei reach close-packing and are quantitatively described by a nonlinear diffusion model. Considerations of nuclear motion constrained inside the enveloping cell membrane show that concentration-dependent stochastic forces inside cells, compatible in magnitude to those found in cytoskeletal transport, can explain the observed magnitude of the diffusion constant.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.58635},
	author = {Azizi, Afnan and Herrmann, Anne and Wan, Yinan and Buse, Salvador Jrp and Keller, Philipp J and Goldstein, Raymond E and Harris, William A}
}
@article {48297,
	title = {The ciliary marginal zone of the zebrafish retina: clonal and time-lapse analysis of a continuously growing tissue.},
	journal = {Development (Cambridge, England)},
	volume = {143},
	year = {2016},
	month = {2016 Apr 01},
	pages = {1099-107},
	abstract = {<p>Clonal analysis is helping us understand the dynamics of cell replacement in homeostatic adult tissues (Simons and Clevers, 2011). Such an analysis, however, has not yet been achieved for continuously growing adult tissues, but is essential if we wish to understand the architecture of adult organs. The retinas of lower vertebrates grow throughout life from retinal stem cells (RSCs) and retinal progenitor cells (RPCs) at the rim of the retina, called the ciliary marginal zone (CMZ). Here, we show that RSCs reside in a niche at the extreme periphery of the CMZ and divide asymmetrically along a radial (peripheral to central) axis, leaving one daughter in the peripheral RSC niche and the other more central where it becomes an RPC. We also show that RPCs of the CMZ have clonal sizes and compositions that are statistically similar to progenitor cells of the embryonic retina and fit the same stochastic model of proliferation. These results link embryonic and postembryonic cell behaviour, and help to explain the constancy of tissue architecture that has been generated over a lifetime.</p>
},
	keywords = {Animals, Animals, Genetically Modified, Cell Differentiation, Cell Division, Cell Proliferation, Gene Expression Regulation, Developmental, Retina, Stem Cells, Zebrafish},
	issn = {1477-9129},
	doi = {10.1242/dev.133314},
	author = {Wan, Yinan and Almeida, Alexandra D and Rulands, Steffen and Chalour, Naima and Muresan, Leila and Wu, Yunmin and Simons, Benjamin D and He, Jie and Harris, William A}
}
