@article {65668,
	title = {Rational design of bioavailable photosensitizers for manipulation and imaging of biological systems.},
	journal = {Cell Chemical Biology},
	volume = {27},
	year = {2020},
	month = {2020 Aug 20},
	pages = {1063-72},
	abstract = {<p>Light-mediated chemical reactions are powerful methods for manipulating and interrogating biological systems. Photosensitizers, compounds that generate reactive oxygen species upon excitation with light, can be utilized for numerous biological experiments, but the repertoire of bioavailable photosensitizers is limited. Here, we describe the synthesis, characterization, and utility of two photosensitizers based upon the widely used rhodamine scaffold and demonstrate their efficacy for chromophore-assisted light inactivation, cell ablation in culture and in\&nbsp;vivo, and photopolymerization of diaminobenzidine for electron microscopy. These chemical tools will facilitate a broad range of applications spanning from targeted destruction of proteins to high-resolution imaging.</p>
},
	issn = {2451-9448},
	doi = {10.1016/j.chembiol.2020.07.001},
	author = {Binns, Thomas C and Ayala, Anthony X and Grimm, Jonathan B and Tkachuk, Ariana N and Castillon, Guillaume A and Phan, Sebastien and Zhang, Lixia and Brown, Timothy A and Liu, Zhe and Adams, Stephen R and Ellisman, Mark H and Koyama, Minoru and Lavis, Luke D}
}
@article {48300,
	title = {Distinct subpopulations of FOXD1 stroma-derived cells regulate renal erythropoietin.},
	journal = {The Journal of Clinical Investigation},
	volume = {126},
	year = {2016},
	month = {2016 May 02},
	pages = {1926-38},
	abstract = {<p>Renal peritubular interstitial fibroblast-like cells are critical for adult erythropoiesis, as they are the main source of erythropoietin (EPO). Hypoxia-inducible factor 2 (HIF-2) controls EPO synthesis in the kidney and liver and is regulated by prolyl-4-hydroxylase domain (PHD) dioxygenases PHD1, PHD2, and PHD3, which function as cellular oxygen sensors. Renal interstitial cells with EPO-producing capacity are poorly characterized, and the role of the PHD/HIF-2 axis in renal EPO-producing cell (REPC) plasticity is unclear. Here we targeted the PHD/HIF-2/EPO axis in FOXD1 stroma-derived renal interstitial cells and examined the role of individual PHDs in REPC pool size regulation and renal EPO output. Renal interstitial cells with EPO-producing capacity were entirely derived from FOXD1-expressing stroma, and Phd2 inactivation alone induced renal Epo in a limited number of renal interstitial cells. EPO induction was submaximal, as hypoxia or pharmacologic PHD inhibition further increased the REPC fraction among Phd2-/- renal interstitial cells. Moreover, Phd1 and Phd3 were differentially expressed in renal interstitium, and heterozygous deficiency for Phd1 and Phd3 increased REPC numbers in Phd2-/- mice. We propose that FOXD1 lineage renal interstitial cells consist of distinct subpopulations that differ in their responsiveness to Phd2 inactivation and thus regulation of HIF-2 activity and EPO production under hypoxia or conditions of pharmacologic or genetic PHD inactivation.</p>
},
	issn = {1558-8238},
	doi = {10.1172/JCI83551},
	author = {Kobayashi, Hanako and Liu, Qingdu and Binns, Thomas C and Urrutia, Andres A and Davidoff, Olena and Kapitsinou, Pinelopi P and Pfaff, Andrew S and Olauson, Hannes and Wernerson, Annika and Fogo, Agnes B and Fong, Guo-Hua and Gross, Kenneth W and Haase, Volker H}
}
