@article {48622,
	title = {Cryo-electron tomography reveals novel features of a viral RNA replication compartment.},
	journal = {eLife},
	volume = {6},
	year = {2017},
	month = {2017 Jun 27},
	abstract = {<p>Positive-strand RNA viruses, the largest genetic class of viruses, include numerous important pathogens such as Zika virus. These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments is largely unknown. We used cryo-electron tomography to reveal many previously unrecognized features of Flock house nodavirus (FHV) RNA replication compartments. These spherular invaginations of outer mitochondrial membranes are packed with electron-dense RNA fibrils and their volumes are closely correlated with RNA replication template length. Each spherule{\textquoteright}s necked aperture is crowned by a striking cupped ring structure containing multifunctional FHV RNA replication protein A. Subtomogram averaging of these crowns revealed twelve-fold symmetry, concentric flanking protrusions, and a central electron density. Many crowns were associated with long cytoplasmic fibrils, likely to be exported progeny RNA. These results provide new mechanistic insights into positive-strand RNA virus replication compartment structure, assembly, function and control.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.25940},
	author = {Ertel, Kenneth J and Benefield, Desir{\'e}e and Casta{\~n}o-Diez, Daniel and Pennington, Janice and Horswill, Mark and den Boon, Johan A and Otegui, Marisa and Ahlquist, Paul}
}
