@article {49056,
	title = {BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis.},
	journal = {Science (New York, N.Y.)},
	volume = {359},
	year = {2018},
	month = {2018 02 23},
	abstract = {<p>Mitochondrial apoptosis is mediated by BAK and BAX, two proteins that induce mitochondrial outer membrane permeabilization, leading to cytochrome c release and activation of apoptotic caspases. In the absence of active caspases, mitochondrial DNA (mtDNA) triggers the innate immune cGAS/STING pathway, causing dying cells to secrete type I interferon. How cGAS gains access to mtDNA remains unclear. We used live-cell lattice light-sheet microscopy to examine the mitochondrial network in mouse embryonic fibroblasts. We found that after BAK/BAX activation and cytochrome c loss, the mitochondrial network broke down and large BAK/BAX pores appeared in the outer membrane. These BAK/BAX macropores allowed the inner mitochondrial membrane to herniate into the cytosol, carrying with it mitochondrial matrix components, including the mitochondrial genome. Apoptotic caspases did not prevent herniation but dismantled the dying cell to suppress mtDNA-induced innate immune signaling.</p>
},
	issn = {1095-9203},
	doi = {10.1126/science.aao6047},
	author = {McArthur, Kate and Whitehead, Lachlan W and Heddleston, John M and Li, Lucy and Padman, Benjamin S and Oorschot, Viola and Geoghegan, Niall D and Chappaz, Stephane and Davidson, Sophia and San Chin, Hui and Lane, Rachael M and Dramicanin, Marija and Saunders, Tahnee L and Sugiana, Canny and Lessene, Romina and Osellame, Laura D and Chew, Teng-Leong and Dewson, Grant and Lazarou, Michael and Ramm, Georg and Lessene, Guillaume and Ryan, Michael T and Rogers, Kelly L and van Delft, Mark F and Kile, Benjamin T}
}
