@article {68631,
	title = {Phase separation of Hippo signalling complexes.},
	journal = {EMBO Journal},
	volume = {42},
	year = {2023},
	month = {2023 Feb 20},
	pages = {e112863},
	abstract = {<p>The Hippo pathway was originally discovered to control tissue growth in Drosophila and includes the Hippo kinase (Hpo; MST1/2 in mammals), scaffold protein Salvador (Sav; SAV1 in mammals) and the Warts kinase (Wts; LATS1/2 in mammals). The Hpo kinase is activated by binding to Crumbs-Expanded (Crb-Ex) and/or Merlin-Kibra (Mer-Kib) proteins at the apical domain of epithelial cells. Here we show that activation of Hpo also involves the formation of supramolecular complexes with properties of a biomolecular condensate, including concentration dependence and\&nbsp;sensitivity to starvation, macromolecular crowding, or 1,6-hexanediol treatment. Overexpressing Ex or Kib induces formation of micron-scale Hpo condensates in the cytoplasm, rather than at the apical membrane. Several Hippo pathway components contain unstructured low-complexity domains and purified Hpo-Sav complexes undergo phase separation in\&nbsp;vitro. Formation of Hpo condensates is conserved in human cells. We propose that apical Hpo kinase activation occurs in phase separated "signalosomes" induced by clustering of upstream pathway components.</p>
},
	issn = {1460-2075},
	doi = {10.15252/embj.2022112863},
	author = {Bonello, Teresa T and Cai, Danfeng and Fletcher, Georgina C and Wiengartner, Kyler and Pengilly, Victoria and Lange, Kimberly S and Liu, Zhe and Lippincott-Schwartz, Jennifer and Kavran, Jennifer M and Thompson, Barry J}
}
@article {49316,
	title = {Apical and basal matrix remodeling control epithelial morphogenesis.},
	journal = {Developmental Cell},
	volume = {46},
	year = {2018},
	month = {2018 Jul 02},
	pages = {23-39.e5},
	abstract = {<p>Epithelial tissues can elongate in two dimensions by polarized cell intercalation, oriented cell division, or cell shape change, owing to local or global actomyosin contractile forces acting in the plane of the tissue. In addition, epithelia can undergo morphogenetic change in three dimensions. We show that elongation of the wings and legs of Drosophila involves a columnar-to-cuboidal cell shape change that reduces cell height and expands cell width. Remodeling of the apical extracellular matrix by the Stubble protease and basal matrix by MMP1/2 proteases induces wing and leg elongation. Matrix remodeling does not occur in the haltere, a limb that fails to elongate. Limb elongation is made anisotropic by planar polarized Myosin-II, which drives convergent extension along the proximal-distal axis. Subsequently, Myosin-II relocalizes to lateral membranes to accelerate columnar-to-cuboidal transition and isotropic tissue expansion. Thus, matrix remodeling induces dynamic changes in actomyosin contractility\&nbsp;to drive epithelial morphogenesis in three dimensions.</p>
},
	issn = {1878-1551},
	doi = {10.1016/j.devcel.2018.06.006},
	author = {Diaz-de-la-Loza, Maria-Del-Carmen and Ray, Robert P and Ganguly, Poulami S and Alt, Silvanus and Davis, John R and Hoppe, Andreas and Tapon, Nic and Salbreux, Guillaume and Thompson, Barry J}
}
