@article {68328,
	title = {Superresolution microscopy reveals actomyosin dynamics in medioapical arrays.},
	journal = {Molecular Biology of the Cell},
	volume = {33},
	year = {2022},
	month = {2022 Sep 15},
	pages = {ar94},
	abstract = {<p>Arrays of actin filaments (F-actin) near the apical surface of epithelial cells (medioapical arrays) contribute to apical constriction and morphogenesis throughout phylogeny. Here, superresolution approaches (grazing incidence structured illumination, GI-SIM, and lattice light sheet, LLSM) microscopy resolve individual, fluorescently labeled F-actin and bipolar myosin filaments that drive amnioserosa cell shape changes during dorsal closure in . In expanded cells, F-actin and myosin form loose, apically domed meshworks at the plasma membrane. The arrays condense as cells contract, drawing the domes into the plane of the junctional belts. As condensation continues, individual filaments are no longer uniformly apparent. As cells expand, arrays of actomyosin are again resolved-some F-actin turnover likely occurs, but a large fraction of existing filaments rearrange. In morphologically isotropic cells, actin filaments are randomly oriented and during contraction are drawn together but remain essentially randomly oriented. In anisotropic cells, largely parallel actin filaments are drawn closer to one another. Our images offer unparalleled resolution of F-actin in embryonic tissue, show that medioapical arrays are tightly apposed to the plasma membrane and are continuous with meshworks of lamellar F-actin. Medioapical arrays thereby constitute modified cell cortex. In concert with other tagged array components, superresolution imaging of live specimens will offer new understanding of cortical architecture and function.</p>
},
	keywords = {Actin Cytoskeleton, Actins, Actomyosin, Animals, Drosophila, Microscopy, Myosins},
	issn = {1939-4586},
	doi = {10.1091/mbc.E21-11-0537},
	author = {Moore, Regan P and Fogerson, Stephanie M and Tulu, U Serdar and Yu, Jason W and Cox, Amanda H and Sican, Melissa A and Li, Dong and Legant, Wesley R and Weigel, Aubrey V and Crawford, Janice M and Betzig, Eric and Kiehart, Daniel P}
}
@article {68078,
	title = {Super-resolution microscopy reveals actomyosin dynamics in medioapical arrays.},
	journal = {Molecular Biology of the Cell},
	year = {2022},
	month = {2022 May 11},
	pages = {mbcE21110537},
	abstract = {<p>Arrays of actin filaments (F-actin) near the apical surface of epithelial cells (medioapical arrays) contribute to apical constriction and morphogenesis throughout phylogeny. Here, super-resolution approaches (grazing incidence structured illumination, GI-SIM and lattice light sheet, LLSM) microscopy resolve individual, fluorescently labeled F-actin and bipolar myosin filaments that drive amnioserosa cell shape changes during dorsal closure in . In expanded cells, F-actin and myosin form loose, apically domed meshworks at the plasma membrane. The arrays condense as cells contract, drawing the domes into the plane of the junctional belts. As condensation continues, individual filaments are no longer uniformly apparent. As cells expand, arrays of actomyosin are again resolved - some F-actin turnover likely occurs, but a large fraction of existing filaments rearrange. In morphologically isotropic cells, actin filaments are randomly oriented and during contraction, are drawn together but remain essentially randomly oriented. In anisotropic cells, largely parallel actin filaments are drawn closer to one another. Our images offer unparalleled resolution of F-actin in embryonic tissue show that medioapical arrays are tightly apposed to the plasma membrane, are continuous with meshworks of lamellar F-actin and thereby constitute modified cell cortex. In concert with other tagged array components, super-resolution imaging of live specimens will offer new understanding of cortical architecture and function. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text].</p>
},
	issn = {1939-4586},
	doi = {10.1091/mbc.E21-11-0537},
	author = {Moore, Regan P and Fogerson, Stephanie M and Tulu, U Serdar and Yu, Jason W and Cox, Amanda H and Sican, Melissa A and Li, Dong and Legant, Wesley R and Weigel, Aubrey V and Crawford, Janice M and Betzig, Eric and Kiehart, Daniel P}
}
@article {49512,
	title = {Visualizing intracellular organelle and cytoskeletal interactions at nanoscale resolution on millisecond timescales. },
	journal = {Cell},
	volume = {175},
	year = {2018},
	month = {2018 Nov 15},
	pages = {1430-42},
	abstract = {In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. To achieve these normally opposing goals, we developed grazing incidence structured illumination microscopy (GI-SIM) that is capable of imaging dynamic events near the basal cell cortex at 97-nm resolution and 266 frames/s over thousands of time points. We employed multi-color GI-SIM to characterize the fast dynamic interactions of diverse organelles and the cytoskeleton, shedding new light on the complex behaviors of these structures. Precise measurements of microtubule growth or shrinkage events helped distinguish among models of microtubule dynamic instability. Analysis of endoplasmic reticulum (ER) interactions with other organelles or microtubules uncovered new ER remodeling mechanisms, such as hitchhiking of the ER on motile organelles. Finally, ER-mitochondria contact sites were found to promote both mitochondrial fission and fusion.},
	doi = {10.1016/j.cell.2018.09.057},
	author = {Guo, Yuting and Li, Di and Zhang, Siwei and Yang, Yanrui and Liu, Jia-Jia and Wang, Xinyu and Liu, Chong and Milkie, Daniel E and Moore, Regan P and Tulu, U Serdar and Kiehart, Daniel P and Hu, Junjie and Lippincott-Schwartz, Jennifer and Betzig, Eric and Li, Dong}
}
