@article {65854,
	title = {Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging.},
	journal = {PLoS Biology},
	volume = {18},
	year = {2020},
	month = {2020 Nov 24},
	pages = {e3000965},
	abstract = {<p>Near-infrared (NIR) genetically encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross talk with visible light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report 2 improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and Caenorhabditis elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca2+ dynamics.</p>
},
	issn = {1545-7885},
	doi = {10.1371/journal.pbio.3000965},
	author = {Qian, Yong and Cosio, Danielle M Orozco and Piatkevich, Kiryl D and Aufmkolk, Sarah and Su, Wan-Chi and Celiker, Orhan T and Schohl, Anne and Murdock, Mitchell H and Abhi Aggarwal and Chang, Yu-Fen and Wiseman, Paul W and Ruthazer, Edward S and Boyden, Edward S and Campbell, Robert E}
}
@article {56204,
	title = {A genetically encoded near-infrared fluorescent calcium ion indicator.},
	journal = {Nature Methods},
	volume = {16},
	year = {2019},
	month = {2019 Jan 21},
	pages = {171-4},
	abstract = {<p>We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca imaging in combination with other optogenetic indicators and actuators.</p>
},
	issn = {1548-7105},
	doi = {10.1038/s41592-018-0294-6},
	author = {Qian, Yong and Piatkevich, Kiryl D and Mc Larney, Benedict and Abdelfattah, Ahmed S and Mehta, Sohum and Murdock, Mitchell H and Gottschalk, Sven and Molina, Rosana S and Zhang, Wei and Chen, Yingche and Wu, Jiahui and Drobizhev, Mikhail and Hughes, Thomas E and Zhang, Jin and Schreiter, Eric R and Shoham, Shy and Razansky, Daniel and Boyden, Edward S and Campbell, Robert E}
}
