@article {64843,
	title = {Split-QF system for fine-tuned transgene expression in Drosophila.},
	journal = {Genetics},
	volume = {212},
	year = {2019},
	month = {2019 Mar 12},
	pages = {53-63},
	abstract = {<p>The Q-system is a binary expression system that works well across species. Here we report the development and demonstrate applications of a split-QF system that drives strong expression in , is repressible by QS and inducible by a small non-toxic molecule quinic acid. The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological and behavioural assays in and in other organisms.</p>
},
	issn = {1943-2631},
	doi = {10.1534/genetics.119.302034},
	author = {Riabinina, Olena and Vernon, Samuel W and Dickson, Barry J and Baines, Richard A}
}
@article {68447,
	title = {A transcriptional reporter of intracellular Ca(2+) in Drosophila.},
	journal = {Nat Neurosci},
	volume = {18},
	year = {2015},
	month = {06/2015},
	pages = {917-25},
	abstract = {<p>Intracellular Ca(2+) is a widely used neuronal activity indicator. Here we describe a transcriptional reporter of intracellular Ca(2+) (TRIC) in Drosophila that uses a binary expression system to report Ca(2+)-dependent interactions between calmodulin and its target peptide. We found that in vitro assays predicted in vivo properties of TRIC and that TRIC signals in sensory systems depend on neuronal activity. TRIC was able to quantitatively monitor neuronal responses that changed slowly, such as those of neuropeptide F-expressing neurons to sexual deprivation and neuroendocrine pars intercerebralis cells to food and arousal. Furthermore, TRIC-induced expression of a neuronal silencer in nutrient-activated cells enhanced stress resistance, providing a proof of principle that TRIC can be used for circuit manipulation. Thus, TRIC facilitates the monitoring and manipulation of neuronal activity, especially those reflecting slow changes in physiological states that are poorly captured by existing methods. TRIC{\textquoteright}s modular design should enable optimization and adaptation to other organisms.</p>
},
	keywords = {Animals, Calcium, Calcium Signaling, Cells, Cultured, Drosophila, Molecular Sequence Data, Mutagenesis, Neural Pathways, Olfactory Pathways, Optic Lobe, Nonmammalian, Stress, Psychological, Synaptic Transmission, Visual Pathways},
	issn = {1546-1726},
	doi = {10.1038/nn.4016},
	author = {Gao, Xiaojing J and Riabinina, Olena and Li, Jiefu and Potter, Christopher J and Clandinin, Thomas R and Luo, Liqun}
}
