@article {64852,
	title = {Cytoskeletal control of antigen-dependent T cell activation.},
	journal = {Cell Reports},
	volume = {26},
	year = {2019},
	month = {2019 Mar 19},
	pages = {3369-3379.e5},
	abstract = {<p>Cytoskeletal actin dynamics is essential for T\&nbsp;cell activation. Here, we show evidence that the binding kinetics of the antigen engaging the T\&nbsp;cell receptor influences the nanoscale actin organization and mechanics of the immune synapse. Using an engineered T\&nbsp;cell system expressing a specific T\&nbsp;cell receptor and stimulated by a range of antigens, we found that the peak force experienced by the T\&nbsp;cell receptor during activation was independent of the unbinding kinetics of the stimulating antigen. Conversely, quantification of the actin retrograde flow velocity at the synapse revealed a striking dependence on the antigen unbinding kinetics. These findings suggest that the dynamics of the actin cytoskeleton actively adjusted to normalize the force experienced by the T\&nbsp;cell receptor in an antigen-specific manner. Consequently, tuning actin dynamics in response to antigen kinetics may thus\&nbsp;be a mechanism that allows T\&nbsp;cells to adjust the lengthscale and timescale of T\&nbsp;cell receptor signaling.</p>
},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2019.02.074},
	author = {Colin-York, Huw and Javanmardi, Yousef and Skamrahl, Mark and Kumari, Sudha and Chang, Veronica T and Khuon, Satya and Taylor, Aaron and Chew, Teng-Leong and Betzig, Eric and Moeendarbary, Emad and Cerundolo, Vincenzo and Eggeling, Christian and Fritzsche, Marco}
}
