@article {45031,
	title = {Transcription factors modulate c-Fos transcriptional bursts.},
	journal = {Cell Reports},
	volume = {8},
	year = {2014},
	month = {2014 Jul 10},
	pages = {75-83},
	abstract = {<p>Transcription is a stochastic process occurring mostly in episodic bursts. Although the local chromatin environment is known to influence the bursting behavior on long timescales, the impact of transcription factors (TFs)-especially in rapidly inducible systems-is largely unknown. Using fluorescence in\&nbsp;situ hybridization and computational models, we quantified the transcriptional activity of the proto-oncogene c-Fos with single mRNA accuracy at individual endogenous alleles. We showed that, during MAPK induction, the TF concentration modulates the burst frequency of c-Fos, whereas other bursting parameters remain mostly unchanged. By using synthetic TFs with TALE DNA-binding domains, we systematically altered different aspects of these bursts. Specifically, we linked the polymerase initiation frequency to the strength of the transactivation domain and the burst duration to the TF lifetime on the promoter. Our results show how TFs and promoter binding domains collectively act to regulate different bursting parameters, offering a vast, evolutionarily tunable regulatory range for individual genes.</p>
},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2014.05.053},
	author = {Senecal, Adrien and Munsky, Brian and Proux, Florence and Ly, Nathalie and Braye, Floriane E and Zimmer, Christophe and Mueller, Florian and Darzacq, Xavier}
}
@article {5522,
	title = {Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.},
	journal = {Nature Methods},
	volume = {10},
	year = {2013},
	month = {2013},
	pages = {60-3},
	abstract = {<p>Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.</p>
},
	issn = {1548-7105},
	doi = {10.1038/nmeth.2277},
	author = {Abrahamsson, Sara and Chen, Jiji and Hajj, Bassam and Stallinga, Sjoerd and Katsov, Alexander Y and Wisniewski, Jan and Mizuguchi, Gaku and Soule, Pierre and Mueller, Florian and Darzacq, Claire Dugast and Darzacq, Xavier and Wu, Carl and Bargmann, Cornelia I and Agard, David A and Dahan, Maxime and Gustafsson, Mats G L}
}
