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A split-GAL4 driver line resource for Drosophila neuron types

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2025 Jul;. doi: 10.7554/elife.98405.2
A split-GAL4 driver line resource for Drosophila neuron types Jayaraman LabRubin LabAso LabBranson LabCellMapFlyEM Project Pipeline SupportProject Technical ResourcesElectron MicroscopyInvertebrate Shared ResourceScientific Computing Software
Meissner GW, Vannan A, Jeter J, Close K, Depasquale GM, Dorman Z, Forster K, Beringer JA, Gibney TV, Hausenfluck JH, He Y, Henderson K, Johnson L, Johnston RM, Ihrke G, Iyer N, Lazarus R, Lee K, Li H, Liaw H, Melton B, Miller S, Motaher R, Novak A, Ogundeyi O, Petruncio A, Price J, Protopapas S, Tae S, Taylor J, Vorimo R, Yarbrough B, Zeng KX, Zugates CT, Dionne H, Angstadt C, Ashley K, Cavallaro A, Dang T, Gonzalez GA, Hibbard KL, Huang C, Kao J, Laverty T, Mercer M, Perez B, Pitts S, Ruiz D, Vallanadu V, Zheng GZ, Goina C, Otsuna H, Rokicki K, Svirskas RR, Cheong HS, Dolan M, Ehrhardt E, Feng K, Galfi B, Goldammer J, Huston SJ, Hu N, Ito M, McKellar C, minegishi r, Namiki S, Nern A, Schretter CE, Sterne GR, Venkatasubramanian L, Wang K, Wolff T, Wu M, George R, Malkesman O, Aso Y, Card GM, Dickson BJ, Korff W, Ito K, Truman JW, Zlatic M, Rubin GM
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Abstract

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Techniques that enable precise manipulations of subsets of neurons in the fly central nervous system have greatly facilitated our understanding of the neural basis of behavior. Split-GAL4 driver lines allow specific targeting of cell types in Drosophila melanogaster and other species. We describe here a collection of 3060 lines targeting a range of cell types in the adult Drosophila central nervous system and 1373 lines characterized in third-instar larvae. These tools enable functional, transcriptomic, and proteomic studies based on precise anatomical targeting. NeuronBridge and other search tools relate light microscopy images of these split-GAL4 lines to connectomes reconstructed from electron microscopy images. The collections are the result of screening over 77,000 split hemidriver combinations. Previously published and new lines are included, all validated for driver expression and curated for optimal cell type specificity across diverse cell types. In addition to images and fly stocks for these well-characterized lines, we make available 300,000 new 3D images of other split-GAL4 lines.

bioRxiv preprint: http://doi.org/10.1101/2024.01.09.574419

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