Bayesian localization microscopy reveals nanoscale podosome dynamics.

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Nature methods. 2012 Feb;9(2):195-200. doi: 10.1038/nmeth.1812

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Bayesian localization microscopy reveals nanoscale podosome dynamics. Lippincott-Schwartz Lab

Cox S, Rosten E, Monypenny J, Jovanovic-Talisman T, Burnette DT, Lippincott-Schwartz J, Jones GE, Heintzmann R

Note: Research in this publication was not performed at Janelia.

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Abstract

We describe a localization microscopy analysis method that is able to extract results in live cells using standard fluorescent proteins and xenon arc lamp illumination. Our Bayesian analysis of the blinking and bleaching (3B analysis) method models the entire dataset simultaneously as being generated by a number of fluorophores that may or may not be emitting light at any given time. The resulting technique allows many overlapping fluorophores in each frame and unifies the analysis of the localization from blinking and bleaching events. By modeling the entire dataset, we were able to use each reappearance of a fluorophore to improve the localization accuracy. The high performance of this technique allowed us to reveal the nanoscale dynamics of podosome formation and dissociation throughout an entire cell with a resolution of 50 nm on a 4-s timescale.

PMID: 22138825 [PubMed - indexed for MEDLINE]

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Bayesian localization microscopy reveals nanoscale podosome dynamics.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 22138825 [PubMed - indexed for MEDLINE]

Janelia Authors