Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway.

janelia7_blocks-janelia7_biblio_header | block

Neuron. 2014 Apr 16;82(2):413-29. doi: 10.1016/j.neuron.2014.02.041

Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway. Looger Lab

Haustein MD, Kracun S, Lu X, Shih T, Jackson-Weaver O, Tong X, Xu J, Yang XW, O’Dell TJ, Marvin JS, Ellisman MH, Bushong EA, Looger LL, Khakh BS

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

The spatiotemporal activities of astrocyte Ca(2+) signaling in mature neuronal circuits remain unclear. We used genetically encoded Ca(2+) and glutamate indicators as well as pharmacogenetic and electrical control of neurotransmitter release to explore astrocyte activity in the hippocampal mossy fiber pathway. Our data revealed numerous localized, spontaneous Ca(2+) signals in astrocyte branches and territories, but these were not driven by neuronal activity or glutamate. Moreover, evoked astrocyte Ca(2+) signaling changed linearly with the number of mossy fiber action potentials. Under these settings, astrocyte responses were global, suppressed by neurotransmitter clearance, and mediated by glutamate and GABA. Thus, astrocyte engagement in the fully developed mossy fiber pathway was slow and territorial, contrary to that frequently proposed for astrocytes within microcircuits. We show that astrocyte Ca(2+) signaling functionally segregates large volumes of neuropil and that these transients are not suited for responding to, or regulating, single synapses in the mossy fiber pathway.

PMID: 24742463 [PubMed - indexed for MEDLINE]

node:body | entity_field

janelia7_blocks-janelia7_biblio_authors | block

janelia7_blocks-janelia7_biblio_tools | block

Main Menu (Mobile)- Block

Main Menu - Block

Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway.

Abstract

PMID: 24742463 [PubMed - indexed for MEDLINE]

Janelia Authors