Direct observation of frequency modulated transcription in single cells using light activation.

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eLife. 2013 Sep 24;2:e00750. doi: 10.7554/eLife.00750

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Direct observation of frequency modulated transcription in single cells using light activation. Singer Lab

Larson DR, Fritzsch C, Sun L, Meng X, Lawrence DS, Singer RH

Note: Research in this publication was not performed at Janelia.

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Abstract

Single-cell analysis has revealed that transcription is dynamic and stochastic, but tools are lacking that can determine the mechanism operating at a single gene. Here we utilize single-molecule observations of RNA in fixed and living cells to develop a single-cell model of steroid-receptor mediated gene activation. We determine that steroids drive mRNA synthesis by frequency modulation of transcription. This digital behavior in single cells gives rise to the well-known analog dose response across the population. To test this model, we developed a light-activation technology to turn on a single steroid-responsive gene and follow dynamic synthesis of RNA from the activated locus. DOI:http://dx.doi.org/10.7554/eLife.00750.001.

PMID: 24069527 [PubMed - indexed for MEDLINE]

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Direct observation of frequency modulated transcription in single cells using light activation.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 24069527 [PubMed - indexed for MEDLINE]

Janelia Authors