Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration.

janelia7_blocks-janelia7_biblio_header | block

bioRxiv. 2021 Jan 21;. doi: https://doi.org/10.1101/2021.01.18.427135

Arts Janine, Mahlandt Eike, Grönloh Max, Schimmel Lilian, Noordstra Ivar, van Steen Abraham, Tol Simon, van Rijssel Jos, Nolte Martijn, Postma Marten, Khuon Satya, Heddleston John, Wait Eric, Chew Teng-Leong, Winter Mark, Montanez Eloi, Goedhart Joachim, van Buul Jaap

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, though it is not yet clear how these hotspots in the endothelium are defined and how they are recognized by the leukocyte. Using lattice light sheet microscopy, we discovered that leukocytes prefer endothelial membrane protrusions at cell junctions for transmigration. Phenotypically, these junctional membrane protrusions are present in an asymmetric manner, meaning that one endothelial cell shows the protrusion and the adjacent one does not. Consequently, leukocytes cross the junction by migrating underneath the protruding endothelial cell. These protrusions depend on Rac1 activity and by using a photo-activatable Rac1 probe, we could artificially generate local exit-sites for leukocytes. Overall, we have discovered a new mechanism that uses local induced junctional membrane protrusions to facilitate/steer the leukocyte escape/exit from inflamed vessel walls.

node:body | entity_field

bioRxiv PrePrint https://doi.org/10.1101/2021.01.18.427135

janelia7_blocks-janelia7_biblio_authors | block

janelia7_blocks-janelia7_biblio_tools | block

Main Menu (Mobile)- Block

Main Menu - Block

Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration.

Abstract

Janelia Authors