Inferring transient particle transport dynamics in live cells.

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Nature Methods. 2015 Jul 20;12(9):838-40. doi: 10.1038/nmeth.3483
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Inferring transient particle transport dynamics in live cells. Singer Lab
Monnier N, Barry Z, Park HY, Su K, Katz Z, English BP, Dey A, Pan K, Cheeseman IM, Singer RH, Bathe M
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Abstract

Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells. The software is available at http://hmm-bayes.org/.

PMID: 26192083 [PubMed - indexed for MEDLINE]

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Janelia Authors

Brian English
Robert Singer
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