Isolation of mitochondria from tissue culture cells.

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Cold Spring Harbor Protocols. 2014 Oct;2014(10):pdb.prot080002. doi: 10.1101/pdb.prot080002
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Isolation of mitochondria from tissue culture cells.
Clayton DA, Shadel GS
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Abstract

The number of mitochondria per cell varies substantially from cell line to cell line. For example, human HeLa cells contain at least twice as many mitochondria as smaller mouse L cells. This protocol starts with a washed cell pellet of 1-2 mL derived from ∼10⁹ cells grown in culture. The cells are swollen in a hypotonic buffer and ruptured with a Dounce or Potter-Elvehjem homogenizer using a tight-fitting pestle, and mitochondria are isolated by differential centrifugation.

PMID: 25275104 [PubMed - indexed for MEDLINE]

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Janelia Authors

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