Photoactivatable drugs for nicotinic optopharmacology.

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Nature Methods. 2018 Mar 26;15(5):347-50. doi: 10.1038/nmeth.4637
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Photoactivatable drugs for nicotinic optopharmacology. Lavis LabJanelia Experimental Technology
Banala S, Arvin MC, Bannon NM, Jin X, Macklin JJ, Wang Y, Peng C, Zhao G, Marshall JJ, Gee KR, Wokosin DL, Kim VJ, McIntosh JM, Contractor A, Lester HA, Kozorovitskiy Y, Drenan RM, Lavis LD
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Abstract

Photoactivatable pharmacological agents have revolutionized neuroscience, but the palette of available compounds is limited. We describe a general method for caging tertiary amines by using a stable quaternary ammonium linkage that elicits a red shift in the activation wavelength. We prepared a photoactivatable nicotine (PA-Nic), uncageable via one- or two-photon excitation, that is useful to study nicotinic acetylcholine receptors (nAChRs) in different experimental preparations and spatiotemporal scales.

PMID: 29578537 [PubMed - indexed for MEDLINE]

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Janelia Authors

Sambashiva Banala
John Macklin
Luke Lavis
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