PSF engineering in multifocus microscopy for increased depth volumetric imaging.

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Biomedical Optics Express. 2016 Feb 16;7(3):726-31. doi: 10.1364/BOE.7.000726
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PSF engineering in multifocus microscopy for increased depth volumetric imaging. Transcription Imaging
Hajj B, Beheiry M, Dahan M
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Abstract

Imaging and localizing single molecules with high accuracy in a 3D volume is a challenging task. Here we combine multifocal microscopy, a recently developed volumetric imaging technique, with point spread function engineering to achieve an increased depth for single molecule imaging. Applications in 3D single molecule localization-based super-resolution imaging is shown over an axial depth of 4 µm as well as for the tracking of diffusing beads in a fluid environment over 8 µm.

PMID: 27231584 [PubMed - indexed for MEDLINE]

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Janelia Authors

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