As-rigid-as-possible mosaicking and serial section registration of large ssTEM datasets.

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Bioinformatics. 2010 Jun 15;26(12):i57-63. doi: 10.1093/bioinformatics/btq219

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As-rigid-as-possible mosaicking and serial section registration of large ssTEM datasets. Cardona LabSaalfeld Lab

Saalfeld S, Cardona A, Hartenstein V, Tomancak P

Note: Research in this publication was not performed at Janelia.

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Abstract

Tiled serial section Transmission Electron Microscopy (ssTEM) is increasingly used to describe high-resolution anatomy of large biological specimens. In particular in neurobiology, TEM is indispensable for analysis of synaptic connectivity in the brain. Registration of ssTEM image mosaics has to recover the 3D continuity and geometrical properties of the specimen in presence of various distortions that are applied to the tissue during sectioning, staining and imaging. These include staining artifacts, mechanical deformation, missing sections and the fact that structures may appear dissimilar in consecutive sections.

PMID: 20529937 [PubMed - indexed for MEDLINE]

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As-rigid-as-possible mosaicking and serial section registration of large ssTEM datasets.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 20529937 [PubMed - indexed for MEDLINE]

Janelia Authors