Single-molecule analysis of gene expression using two-color RNA labeling in live yeast.

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Nature Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305

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Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. Singer Lab

Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH

Note: Research in this publication was not performed at Janelia.

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Abstract

Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene.

PMID: 23263691 [PubMed - indexed for MEDLINE]

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Single-molecule analysis of gene expression using two-color RNA labeling in live yeast.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 23263691 [PubMed - indexed for MEDLINE]

Janelia Authors