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janelia7_blocks-janelia7_biblio_header | block
Optics Letters. 2008 Jul 15;33(14):1629-31
Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator.
Fiolka R, Beck M, Stemmer A
Note: Research in this publication was not performed at Janelia.
janelia7_blocks-janelia7_biblio_abstract | block
Abstract
In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.
PMID: 18628820 [PubMed - indexed for MEDLINE]
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janelia7_blocks-janelia7_biblio_tools | block