Three-dimensional preparation and imaging reveal intrinsic microtubule properties.

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Nature Methods. 2007 Oct;4(10):843-6. doi: 10.1038/nmeth1087

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Three-dimensional preparation and imaging reveal intrinsic microtubule properties. Keller Lab

Keller PJ, Pampaloni F, Stelzer EH

Note: Research in this publication was not performed at Janelia.

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Abstract

We present an experimental investigation of microtubule dynamic instability in three dimensions, based on laser light-sheet fluorescence microscopy. We introduce three-dimensional (3D) preparation of Xenopus laevis egg extracts in Teflon-based cylinders and provide algorithms for 3D image processing. Our approach gives experimental access to the intrinsic dynamic properties of microtubules and to microtubule population statistics in single asters. We obtain evidence for a stochastic nature of microtubule pausing.

PMID: 17828271 [PubMed - indexed for MEDLINE]

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Three-dimensional preparation and imaging reveal intrinsic microtubule properties.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 17828271 [PubMed - indexed for MEDLINE]

Janelia Authors