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Unlimited genetic switches for cell-type-specific manipulation.

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Neuron. 2019 Oct 23;104(2):227-38. doi: https://doi.org/10.1016/j.neuron.2019.07.005
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Unlimited genetic switches for cell-type-specific manipulation. Espinosa Medina Lab
Garcia-Marques J, Yang C, Espinosa Medina Isabel, Mok K, Koyama M, Lee T
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Abstract

Gaining independent genetic access to discrete cell types is critical to interrogate their biological functions as well as to deliver precise gene therapy. Transcriptomics has allowed us to profile cell populations with extraordinary precision, revealing that cell types are typically defined by a unique combination of genetic markers. Given the lack of adequate tools to target cell types based on multiple markers, most cell types remain inaccessible to genetic manipulation. Here we present CaSSA, a platform to create unlimited genetic switches based on CRISPR/Cas9 (Ca) and the DNA repair mechanism known as single-strand annealing (SSA). CaSSA allows engineering of independent genetic switches, each responding to a specific gRNA. Expressing multiple gRNAs in specific patterns enables multiplex cell-type-specific manipulations and combinatorial genetic targeting. CaSSA is a new genetic tool that conceptually works as an unlimited number of recombinases and will facilitate genetic access to cell types in diverse organisms.

PMID: 31395429 [PubMed - indexed for MEDLINE]

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Previously published as bioRxiv Preprint  https://doi.org/10.1101/470443 (November 14, 2018)

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Janelia Authors

Isabel Espinosa Medina
Minoru Koyama
Tzumin Lee
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