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Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.

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Nature Methods. 2008 Jan;5(1):49-51. doi: 10.1038/nmeth1146
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Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Rubin Lab
Ni J, Markstein M, Binari R, Pfeiffer B, Liu L, Villalta C, Booker M, Perkins L, Perrimon N
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Abstract

The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.

PMID: 18084299 [PubMed - indexed for MEDLINE]

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