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janelia7_blocks-janelia7_biblio_header | block
Nature Methods. 2008 Jan;5(1):49-51. doi: 10.1038/nmeth1146
Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Rubin Lab
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Ni J, Markstein M, Binari R, Pfeiffer B, Liu L, Villalta C, Booker M, Perkins L, Perrimon N
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Abstract
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
PMID: 18084299 [PubMed - indexed for MEDLINE]
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janelia7_blocks-janelia7_biblio_authors | block
Janelia Authors
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