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Histochemistry and Cell Biology. 2007 Dec;128(6):499-505. doi: 10.1007/s00418-007-0342-2
Virtual slit scanning microscopy.
Fiolka R, Stemmer A, Belyaev Y
Note: Research in this publication was not performed at Janelia.
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Abstract
We present a novel slit scanning confocal microscope with a CCD camera image sensor and a virtual slit aperture for descanning that can be adjusted during post-processing. A very efficient data structure and mathematical criteria for aligning the virtual aperture guarantee the ease of use. We further introduce a method to reduce the anisotropic lateral resolution of slit scanning microscopes. System performance is evaluated against a spinning disk confocal microscope on identical specimens. The virtual slit scanning microscope works as the spinning disk type and outperforms on thick specimens.
PMID: 17891411 [PubMed - indexed for MEDLINE]
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