What We Do

Our Gene Targeting & Transgenic Facility makes sophisticated gene targeting a turnkey service at a low cost. We develop and experiment with new technologies to support research needs. We design and produce gene targeting, CRISPR/Cas9 and BAC transgenic constructs; we generate gene targeting animals using ES cell approach for the mouse and CRISPR/Cas9 and integrase approaches for the mouse and the rat; we cryopreserve and recover genetically modified mouse and rat lines.

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Services

Design and generate gene targeting constructs, including knockin report genes, knockin mutations, conventional/conditional knockouts, and BAC transgenics and constructs for CRISPR/cas9 targeting
ES cell targeting
Generation of chimeras through ES cell/embryo aggregation
DNA pronuclear microinjection and CRISPR/cas9 microinjection
Establish new ES cell lines from blastocysts
Mouse Embryo/sperm Cryopreservation and IVF to recover the lines
Developing new technologies

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Gene Targeting and Transgenics

What We Do

Services