Videos of our Techniques
These videos illustrate FlyLight methods of dissecting and mounting the central nervous system of larval and adult Drosophila. Videos by FlyLight Team.
Adult Brain Dissection
The brain is removed from an anesthetized Drosophila melanogaster adult.
Adult CNS dissection
The central nervous system (brain and ventral nerve cord) is removed from an anesthetized Drosophila melanogaster adult.
Adult Mounting
Fixed adult Drosophila melanogaster central nervous systems are mounted on a poly-L-lysine coated coverslip (see Recipe - Poly-L-Lysine), using a mounting dish described on the Drosophila Mounting T-Dish page.
DPX Embedding
A coverslip with mounted central nervous systems is embedded in DPX on a glass slide (see DPX Mounting).
Larval Dissection
The central nervous system is removed from a Drosophila melanogaster third instar larva.
Larval Mounting
Fixed larval Drosophila melanogaster central nervous systems are mounted on a poly-L-lysine coated coverslip (see Recipe - Poly-L-Lysine), using a mounting dish described on the Drosophila Mounting T-Dish page.
Download our Protocols
Adult Drosophila CNS
Dissection and Fixation 1.2% PFA (PDF)
Dissection and Fixation 2% PFA (PDF)
Glycerol Mounting (PDF)
DPX Mounting (PDF)
IHC - Adult Split Screen (PDF)
IHC - Anti-GFP (PDF)
IHC - Double Label (PDF)
IHC - MCFO (PDF)
IHC - Polarity Sequential (PDF)
IHC - Polarity Sequential Case 5 (PDF)
Hybrid IHC/Chemical Tag - MCFO (PDF)
Recipe - PFA (PDF)
Recipe - Poly-L-Lysine (PDF)
Larval Drosophila CNS
Dissection and Fixation 4% PFA (PDF)
IHC - MCFO (PDF)
IHC - Polarity (PDF)
IHC-Split-GAL4 (Anti-GFP) (PDF)