Approaches toward super-resolution fluorescence imaging of mitochondrial proteins using PALM.

Our Research / Publications

janelia7_blocks-janelia7_biblio_header | block

Methods. 2010 Aug;51(4):458-63. doi: 10.1016/j.ymeth.2010.01.001

View Article on

Approaches toward super-resolution fluorescence imaging of mitochondrial proteins using PALM. Fetter Lab

Brown TA, Fetter RD, Tkachuk AN, Clayton DA

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Mitochondria are difficult targets for microscopy because of their small size and highly compartmentalized, membranous interior. Super-resolution fluorescence microscopy methods have recently been developed that exceed the historical limits of optical imaging. Here we outline considerations and techniques in preparing to image the relative location of mitochondrial proteins using photoactivated localization microscopy (PALM). PALM and similar methods have the capacity to dramatically increase our ability to image proteins within mitochondria, and to expand our knowledge of the location of macromolecules beyond the current limits of immunoEM.

PMID: 20060907 [PubMed - indexed for MEDLINE]

node:body | entity_field

Main Menu (Mobile)- Block

Main Menu - Block

Approaches toward super-resolution fluorescence imaging of mitochondrial proteins using PALM.

Abstract

PMID: 20060907 [PubMed - indexed for MEDLINE]

Janelia Authors