Approaches toward super-resolution fluorescence imaging of mitochondrial proteins using PALM.

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Methods. 2010 Aug;51(4):458-63. doi: 10.1016/j.ymeth.2010.01.001
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Approaches toward super-resolution fluorescence imaging of mitochondrial proteins using PALM. Fetter Lab
Brown TA, Fetter RD, Tkachuk AN, Clayton DA
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Abstract

Mitochondria are difficult targets for microscopy because of their small size and highly compartmentalized, membranous interior. Super-resolution fluorescence microscopy methods have recently been developed that exceed the historical limits of optical imaging. Here we outline considerations and techniques in preparing to image the relative location of mitochondrial proteins using photoactivated localization microscopy (PALM). PALM and similar methods have the capacity to dramatically increase our ability to image proteins within mitochondria, and to expand our knowledge of the location of macromolecules beyond the current limits of immunoEM.

PMID: 20060907 [PubMed - indexed for MEDLINE]

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Janelia Authors

Ariana Tkachuk
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