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Nature Methods. 2020 Mar 02;17(3):291-4. doi: 10.1038/s41592-020-0760-9
Rapid mesoscale volumetric imaging of neural activity with synaptic resolution. Svoboda LabJanelia Experimental Technology
Lu R, Liang Y, Meng G, Zhou P, Svoboda K, Paninski L, Ji N
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Abstract
Imaging neurons and neural circuits over large volumes at high speed and subcellular resolution is a difficult task. Incorporating a Bessel focus module into a two-photon fluorescence mesoscope, we achieved rapid volumetric imaging of neural activity over the mesoscale with synaptic resolution. We applied the technology to calcium imaging of entire dendritic spans of neurons as well as neural ensembles within multiple cortical regions over two hemispheres of the awake mouse brain.
PMID: 32123393 [PubMed - indexed for MEDLINE]
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Janelia Authors
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