Molecular Genomics

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More in this Support Team
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What We Do

The Molecular Genomics team offers services and expert advice on routine and specialized molecular biology techniques.

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Equipment

  • Andrew plus pipetting robot

  • Bio-Rad ChemiDoc imaging station

  • Bio-Rad Gel Electrophoresis

  • Bio-Rad T100 Thermal cyclers

  • Bio-Rad Western Blot transfer

  • Eppendorf Gradient Thermal cycler

  • Helix agarose gel electrophoresis stations

  • QIAGEN QIAxcel Connect capillary electrophoresis

  • QIAGEN QIAcuity Digital PCR System

  • Single channel Nanodrops

  • ThermoFisher 2720 Thermal cyclers

  • Vizgen MERSCOPE

Services

Plasmid Prep Service

  • Endotoxin-free maxi plasmid preps

  • Whole plasmid sequencing with outside providers

Custom Cloning

  • Design, cloning, and validation of custom vectors using both in-house expertise and outside service providers

Genome Engineering

  • Design and prepare guideRNA, Cas enzyme, and homology-directed repair donor templates

  • Coordinate with non-rodent shared resources for delivery to embryo

  • Coordinate with shared resources for cell-line development

  • Mutation detection and identification

Spatial Transcriptomics

  • Vizgen MERSCOPE sample hybridization with probes and imaging

Resources

  • qPCR and RT-qPCR gene expression analysis with commercial or custom assays
  • Protein electrophoreses and Western blot analysis
  • High volume protein production with outside providers
  • Commonly used vectors for virus production, protein expression, and fluorescent protein labeling
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