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What We Do
The Molecular Genomics team offers services and expert advice on routine and specialized molecular biology techniques.
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Equipment
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Andrew plus pipetting robot
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Bio-Rad ChemiDoc imaging station
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Bio-Rad Gel Electrophoresis
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Bio-Rad T100 Thermal cyclers
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Bio-Rad Western Blot transfer
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Eppendorf Gradient Thermal cycler
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Helix agarose gel electrophoresis stations
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Single and multichannel Nanodrops
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ThermoFisher 2720 Thermal cyclers
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Vizgen MERSCOPE
Services
Plasmid Prep Service
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Endotoxin-free maxi plasmid preps
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Whole plasmid sequencing with outside providers
Custom Cloning
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Design, cloning, and validation of custom vectors using both in-house expertise and outside service providers
Genome Engineering
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Design and prepare guideRNA, Cas enzyme, and homology-directed repair donor templates
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Coordinate with non-rodent shared resources for delivery to embryo
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Coordinate with shared resources for cell-line development
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Mutation detection and identification
Spatial Transcriptomics
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Vizgen MERSCOPE sample hybridization with probes and imaging
Resources
- qPCR and RT-qPCR gene expression analysis with commercial or custom assays
- Protein electrophoreses and Western blot analysis
- High volume protein production with outside providers
- Commonly used vectors for virus production, protein expression, and fluorescent protein labeling
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