Light-Based Approaches to Neural Circuit Reconstruction
New genetic, molecular, optical and computational tools are transforming light microscopy in ways that offer the promise of neural circuit reconstruction at previously inconceivable levels of detail and completeness. This workshop explored current advances in light-based approaches to high-resolution neural circuit imaging and analysis, including:
- genetic methods (targeted gene expression, recombinase-based stochastic labeling, fluorescent proteins)
- cytochemistry (indicators and proteomic reagents)
- light-based acquisition strategies (nanoscopy, array tomography, multi-photon imaging)
- informatics (registration, tracing, visualization, modeling, integration of light and electron microscopic data).
We also had a session devoted to some of the high-throughput programs currently underway or in the planning stages.
Organizers
Jeff Lichtman, Harvard University
Gene Myers, Janelia Research Campus/HHMI
Gerry Rubin, Janelia Research Campus/HHMI
Stephen Smith, Stanford University
Invited Participants
Douglas Armstrong, University of Edinburgh
Herwig Baier, University of California, San Francisco
Eric Betzig, Janelia Research Campus/HHMI
Sydney Brenner, Janelia Research Campus/HHMI
Randy Bruno, Columbia University
Andreas Burkhalter, Washington University in St. Louis
Dawen Cai, Harvard University
Albert Cardona, University of Zurich and ETH Zurich
Dmitri Chklovskii, Janelia Research Campus/HHMI
Thomas Clandinin, Stanford University
Hollis Cline, The Scripps Research Institute
Winfried Denk, Max-Planck-Institute for Medical Research
David DiGregorio, The European Neuroscience Institute
Lynn Enquist, Princeton University
Guoping Feng, Massachusetts Institute of Technology
Seth Grant, The Wellcome Trust Sanger Institute
Tim Harris, Janelia Research Campus/HHMI
Kristen Harris, University of Texas, Austin
Michael Hausser, University College London
Michael Hawrylycz, Allen Institute for Brain Science
Harald Hess, Janelia Research Campus/HHMI
Z. Josh Huang, Cold Spring Harbor Laboratory
Gregory Jefferis, MRC Laboratory of Molecular Biology
Andreas Jeromin, Allen Institute for Brain Science
Tzumin Lee, Janelia Research Campus/HHMI
Loren Looger, Janelia Research Campus/HHMI
Robert Marc, University of Utah School of Medicine
Kristina Micheva, Stanford University
Partha Mitra, Cold Spring Harbor Laboratory
Atsushi Miyawaki, RIKEN Brain Science Institute
Josh Morgan, University of Washington
Sacha Nelson, Brandeis University
Aljoscha Nern, Janelia Research Campus/HHMI
Pavel Osten, Cold Spring Harbor Laboratory
Hanchuan Peng, Janelia Research Campus/HHMI
Badri Roysam, Rennselaer Polytechnic Institute
Alexander Schier, Harvard University
Sebastian Seung, HHMI/Massachusetts Institute of Technology
Kang Shen, HHMI/Stanford University
Charles Stevens, Salk Institute
Michael Stryker, University of California, San Francisco
Karel Svoboda, Janelia Research Campus/HHMI
James Truman, Janelia Research Campus/HHMI
Roger Tsien, HHMI/University of California, San Diego
Ian Wickersham, Massachusetts Institute of Technology
Hongkui Zeng, Allen Institute for Brain Science
Xiaowei Zhuang, HHMI/Harvard University
Larry Zipursky, HHMI/University of California, Los Angeles
Yi Zuo, University of California, Santa Cruz
