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Project Teams:
- Overview
- Anatomy and Histology
- Cryo-Electron Microscopy
- Electron Microscopy
- Flow Cytometry
- Gene Targeting and Transgenics
- Immortalized Cell Line Culture
- Integrative Imaging
- Invertebrate Shared Resource
- Janelia Experimental Technology
- Mass Spectrometry
- Media Prep
- Molecular Genomics
- Primary & iPS Cell Culture
- Project Pipeline Support
- Project Technical Resources
- Quantitative Genomics
- Scientific Computing Software
- Scientific Computing Systems
- Viral Tools
- Vivarium
2025
Renganathan B, Moore AS, Yeo W-H, Petruncio A, Ackerman D, Weigel AV, Team TCellMap, H. Pasolli A, C. Xu S, Shtengel G et al..
2025. Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM. Journal of Cell Biology. 224:e202406054.
2024
Liao Y-C, Pang S, Li W-P, Shtengel G, Choi H, Schaefer K, C Xu S, Lippincott-Schwartz J.
2024. COPII with ALG2 and ESCRTs control lysosome-dependent microautophagy of ER exit sites.. Dev Cell.
Obara CJ, Nixon-Abell J, Moore AS, Riccio F, Hoffman DP, Shtengel G, C Xu S, Schaefer K, H Pasolli A, Masson J-B et al..
2024. Motion of VAPB molecules reveals ER-mitochondria contact site subdomains.. Nature. 626(7997):169-176.
2022
Ritter AT, Shtengel G, C Xu S, Weigel A, Hoffman DP, Freeman M, Iyer N, Alivodej N, Ackerman D, Voskoboinik I et al..
2022. ESCRT-mediated membrane repair protects tumor-derived cells against T cell attack.. Science. 376(6591):377-382.
2021
Weigel AV, Chang C-L, Shtengel G, C Xu S, Hoffman DP, Freeman M, Iyer N, Aaron J, Khuon S, Bogovic J et al..
2021. ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER.. Cell. 184(9):2412.
C Xu S, Pang S, Shtengel G, Müller A, Ritter AT, Hoffman HK, Takemura S-ya, Lu Z, H Pasolli A, Iyer N et al..
2021. An open-access volume electron microscopy atlas of whole cells and tissues.. Nature. 599(7883):147-51.
2020
Hoffman DP, Shtengel G, C Xu S, Campbell KR, Freeman M, Wang L, Milkie DE, H Pasolli A, Iyer N, Bogovic JA et al..
2020. Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells.. Science. 367(6475)
2019
Chang C-L, Weigel AV, Ioannou MS, H Pasolli A, C Xu S, Peale DR, Shtengel G, Freeman M, Hess HF, Blackstone C et al..
2019. Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III.. Journal of Cell Biology. 218(8):2583-99.
2017
Kopek BG, Paez-Segala MG, Shtengel G, Sochacki KA, Sun MG, Wang Y, C Xu S, Van Engelenburg SB, Taraska JW, Looger LL et al..
2017. Diverse protocols for correlative super-resolution fluorescence imaging and electron microscopy of chemically fixed samples.. Nature Protocols. 12(5):916-946.
2015
Hell SW, Sahl SJ, Bates M, Zhuang X, Heintzmann R, Booth MJ, Bewersdorf J, Shtengel G, Hess HF, Tinnefeld P.
2015. The 2015 super-resolution microscopy roadmap.. Journal of Physics D: Applied Physics. 48:443001.
Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin JJ, Patel R, Allen JR, Howe ES, Piszczek G et al..
2015. Fixation-resistant photoactivatable fluorescent proteins for CLEM.. Nature Methods. 12(3):215-8.
Case LB, Baird MA, Shtengel G, Campbell SL, Hess HF, Davidson MW, Waterman CM.
2015. Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions.. Nature Cell Biology. 17(7):880-92.
Buss J, Coltharp C, Shtengel G, Yang X, Hess H, Xiao J.
2015. A multi-layered protein network stabilizes the Escherichia coli FtsZ-ring and modulates constriction dynamics.. PLoS Genetics. 11(4):e1005128.
Grimm JB, Klein T, Kopek BG, Shtengel G, Hess HF, Sauer M, Lavis LD.
2015. Synthesis of a far-red photoactivatable silicon-containing rhodamine for super-resolution microscopy.. Angewandte Chemie (International ed. in English). 55(5):1723-7.
2014
Lee D, Shtengel G, Osborne JE, Lee AK.
2014. Anesthetized- and awake-patched whole-cell recordings in freely moving rats using UV-cured collar-based electrode stabilization.. Nature Protocols. 9(12):2784-95.
Sochacki KA, Shtengel G, Van Engelenburg SB, Hess HF, Taraska JW.
2014. Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.. Nature Methods. 11(3):305-8.
Van Engelenburg SB, Shtengel G, Sengupta P, Waki K, Jarnik M, Ablan SD, Freed EO, Hess HF, Lippincott-Schwartz J.
2014. Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits.. Science. 343(6171):653-6.
Van Engelenburg SB, Shtengel G, Sengupta P, Waki K, Jarnik M, Ablan SD, Freed EO, Hess HF, Lippincott-Schwartz J.
2014. Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits.. Science (New York, N.Y.). 343(6171):653-6.
Shtengel G, Wang Y, Zhang Z, Goh WIng, Hess HF, Kanchanawong P.
2014. Imaging cellular ultrastructure by PALM, iPALM, and correlative iPALM-EM.. Methods in Cell Biology. 123:273-94.
2013
Kopek BG, Shtengel G, Grimm JB, Clayton DA, Hess HF.
2013. Correlative photoactivated localization and scanning electron microscopy.. PLoS One. 8(10):e77209.
2012
Kopek BG, Shtengel G, C Xu S, Clayton DA, Hess HF.
2012. Correlative 3D superresolution fluorescence and electron microscopy reveal the relationship of mitochondrial nucleoids to membranes.. Proceedings of the National Academy of Science of the United States of America. 109(16):6136-41.
Sochacki KA, Larson BT, Sengupta DC, Daniels MP, Shtengel G, Hess HF, Taraska JW.
2012. Imaging the post-fusion release and capture of a vesicle membrane protein.. Nature Communications. 3:1154.
2011
Brown TA, Tkachuk AN, Shtengel G, Kopek BG, Bogenhagen DF, Hess HF, Clayton DA.
2011. Superresolution fluorescence imaging of mitochondrial nucleoids reveals their spatial range, limits, and membrane interaction.. Molecular and Cellular Biology. 31:4994-5010.
2010
Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess HF, Waterman CM.
2010. Nanoscale architecture of integrin-based cell adhesions.. Nature. 468(7323):580-4.
2009
Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, Davidson MW et al..
2009. Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure.. Proceedings of the National Academy of Sciences of the United States of America. 106:3125-30.