An adaptive optics module for deep tissue multiphoton imaging in vivo

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Nature Methods. 2021 Oct 04;:1259-64. doi: 10.1038/s41592-021-01279-0
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An adaptive optics module for deep tissue multiphoton imaging in vivo
Rodríguez Cristina, Chen Anderson, Rivera José, Mohr Manuel, liang Yajie, Sun Wenzhi, Milkie Daniel, Bifano Thomas, Chen Xiaoke, Ji Na
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Abstract

Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at unprecedented depths in vivo.

PMID: 34608309 [PubMed - indexed for MEDLINE]

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bioRxiv PrePrint https://doi.org/10.1101/2020.11.25.397968

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Janelia Authors

Daniel Milkie
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