An adaptive optics module for deep tissue multiphoton imaging in vivo.

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Nature Methods. 2021 Oct 01;18(10):1259-1264. doi: 10.1038/s41592-021-01279-0
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An adaptive optics module for deep tissue multiphoton imaging in vivo. Janelia Experimental Technology
Rodriguez C, Chen A, Rivera JA, Mohr MA, Liang Y, Natan RG, Sun W, Milkie DE, Bifano TG, Chen X, Ji N
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Abstract

Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.

PMID: 34608309 [PubMed - indexed for MEDLINE]

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Janelia Authors

Alex Chen
Yajie Liang
Daniel Milkie
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